While both bacteria and fungi are important for the degradation and humification of organic matter during composting, it is unclear to what extent their roles are associated with abiotic compost properties. This study evaluated changes in abiotic compost properties and the succession of bacterial and fungal communities during pig manure composting for 90 days. The compost rapidly reached thermophilic phase (>58 ℃), which lasted for 15 days. Both bacterial and fungal community compositions changed drastically during composting and while bacterial diversity increased, the fungal diversity decreased during the thermophilic phase of composting. Two taxa dominated both bacterial (Bacillales and Clostridiales) and fungal (Eurotiales and Glomerellales) communities and these showed alternating abundance fluctuations following different phases of composting. The abundance fluctuations of most dominant bacterial and fungal taxa could be further associated with decreases in the concentrations of fulvic acid, cellulose, hemicellulose and overall biodegradation potential in the compost. Moreover, bacterial predicted metabolic gene abundances dominated the first three phases of composting, while predicted fungal saprotrophic functional genes increased consistently, reaching highest abundances towards the end of composting. Finally, redundancy analysis (RDA) showed that changes in abiotic compost properties correlated with the bacterial community diversity and carbohydrate metabolism and fungal wood saprotrophic function. Together these results suggests that bacterial and fungal community succession was associated with temporal changes in abiotic compost properties, potentially explaining alternating taxa abundance patterns during pig manure composting.

虽然细菌和真菌对于堆肥过程中有机物的降解和腐殖化都很重要，但尚不清楚它们的作用在多大程度上与非生物堆肥特性相关。本研究评估了 90 天猪粪堆肥过程中非生物堆肥特性的变化以及细菌和真菌群落的演替。堆肥迅速达到嗜热阶段（>58℃），持续15天。在堆肥过程中细菌和真菌群落组成都发生了巨大变化，虽然细菌多样性增加，但在堆肥的高温阶段真菌多样性下降。两个类群为主的两种细菌（芽孢杆菌和梭菌）和真菌（散囊菌和Glomerellales) 群落，这些群落在不同的堆肥阶段后显示出交替的丰度波动。大多数优势细菌和真菌类群的丰度波动可能进一步与堆肥中富里酸、纤维素、半纤维素和整体生物降解潜力的浓度降低有关。此外，细菌预测的代谢基因丰度主导了堆肥的前三个阶段，而预测的真菌腐生功能基因持续增加，在堆肥结束时达到最高丰度。最后，冗余分析 (RDA) 表明，非生物堆肥特性的变化与细菌群落多样性、碳水化合物代谢和真菌木材腐生功能相关。