Dennentia tripetalla (Pepper Fruit) belongs to the Annonaceae family and is abundant in Nigeria. Its fruit in folklore medicine is used for treatment of varying ailments. While ample research evidence exists on the plants fruit and seed, no current study exists on the toxicological profile of the plant leaves. qualitative and quantitative phytochemicals and In vitro antioxidant assays were carried out using standard methods. The acute toxicity study indicates that the LD50 was higher than 2000 mg/Kg body weight. Sub-chronic toxicity studies was carried out using five groups of rats. Group 1 served as control, 2–5 received 100 mg/Kg, 200 mg/Kg, 500 mg/Kg and 1000 mg/Kg body weight orally for 28 days. Post-administration biochemical analysis indicates there was increased weight in rats administered 100 mg/kg and 200 mg/kg while it reduced in the 500 mg/kg group. Significant elevations of liver function markers were reported for 200 mg/kg and 500 mg/kg respectively. Serum and hepatic protein profiles remained unaltered. Renal function analysis revealed elevated serum urea and creatinine for 200 and 500 mg/kg groups, elevated serum Na+ and Ca+ and reduced serum Cl− for the 500 mg/Kg group. Elevated Kidney K+ and Ca+ levels, reduced Cl− were significantly observed in 500 mg/Kg group. Significant rise in hepatic and renal lipid peroxidation was observed in 200 and 500 mg/Kg groups. There were observed disarmament of the antioxidant defense systems occasioned by rise and drop in tissue (hepatic, renal, testes, heart) Superoxide dismutase (SOD), Catalase (Cat), Glutathione-s-transferase (GST), Glutathione peroxidase (GPx) activities in the test groups relative to control. Histopathological examination indicated architectural aberrations at 500 and 1000 mg/kg. It concluded that the plant had significant phytochemical and antioxidant properties of medical interest and possessed toxic properties in rats when administered at a dose above 200 mg/Kg over a prolonged period of time.

中文翻译：

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Dennettia tripetala (Pepper Fruit) 甲醇叶提取物的初步毒理学特征

Dennentia tripetalla (Pepper Fruit) 属于番荔枝科，在尼日利亚盛产。它在民间医学中的果实用于治疗各种疾病。虽然有大量关于植物果实和种子的研究证据，但目前没有关于植物叶子毒理学特征的研究。使用标准方法进行定性和定量植物化学物质和体外抗氧化剂测定。急性毒性研究表明 LD50 高于 2000 mg/Kg 体重。使用五组大鼠进行亚慢性毒性研究。第 1 组作为对照，第 2-5 组口服 100 毫克/公斤、200 毫克/公斤、500 毫克/公斤和 1000 毫克/公斤体重，持续 28 天。给药后生化分析表明，给药 100 mg/kg 和 200 mg/kg 的大鼠体重增加，而给药 500 mg/kg 组的大鼠体重减少。分别报告了 200 mg/kg 和 500 mg/kg 的肝功能标志物显着升高。血清和肝脏蛋白质谱保持不变。肾功能分析显示，200 和 500 mg/kg 组的血清尿素和肌酐升高，500 mg/Kg 组的血清 Na+ 和 Ca+ 升高，血清 Cl− 降低。在 500 mg/Kg 组中显着观察到肾脏 K+ 和 Ca+ 水平升高，Cl− 降低。在 200 和 500 mg/Kg 组中观察到肝和肾脂质过氧化显着升高。观察到由组织（肝、肾、睾丸、心脏）超氧化物歧化酶 (SOD) 的上升和下降引起的抗氧化防御系统的解除，与对照组相比，测试组中的过氧化氢酶 (Cat)、谷胱甘肽-s-转移酶 (GST)、谷胱甘肽过氧化物酶 (GPx) 活性。组织病理学检查表明在 500 和 1000 mg/kg 时出现结构畸变。它的结论是，该植物具有显着的医学意义的植物化学和抗氧化特性，并且当以超过 200 毫克/公斤的剂量长期给药时，对大​​鼠具有毒性。