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Comparison of coenzyme Q10 or fish oil for prevention of intermittent hypoxia-induced oxidative injury in neonatal rat lungs
Respiratory Research ( IF 4.7 ) Pub Date : 2021-07-05 , DOI: 10.1186/s12931-021-01786-w
Christina D'Agrosa 1 , Charles L Cai 1 , Faisal Siddiqui 1 , Karen Deslouches 1 , Stephen Wadowski 1 , Jacob V Aranda 1, 2, 3 , Kay D Beharry 1, 2, 3
Affiliation  

Neonatal intermittent hypoxia (IH) results in oxidative distress in preterm infants with immature antioxidant systems, contributing to lung injury. Coenzyme Q10 (CoQ10) and fish oil protect against oxidative injury. We tested the hypothesis that CoQ10 is more effective than fish oil for prevention of IH-induced lung injury in neonatal rats. Newborn rats were exposed to two clinically relevant IH paradigms at birth (P0): (1) 50% O2 with brief hypoxia (12% O2); or (2) room air (RA) with brief hypoxia (12% O2), until P14 during which they were supplemented with daily oral CoQ10, fish oil, or olive oil from P0 to P14. Pups were studied at P14 or placed in RA until P21 with no further treatment. Lungs were assessed for histopathology and morphometry; biomarkers of oxidative stress and lipid peroxidation; and antioxidants. Of the two neonatal IH paradigms 21%/12% O2 IH resulted in the most severe outcomes, evidenced by histopathology and morphometry. CoQ10 was effective for preserving lung architecture and reduction of IH-induced oxidative stress biomarkers. In contrast, fish oil resulted in significant adverse outcomes including oversimplified alveoli, hemorrhage, reduced secondary crest formation and thickened septae. This was associated with elevated oxidants and antioxidants activities. Data suggest that higher FiO2 may be needed between IH episodes to curtail the damaging effects of IH, and to provide the lungs with necessary respite. The negative outcomes with fish oil supplementation suggest oxidative stress-induced lipid peroxidation.

中文翻译:

辅酶Q10或鱼油预防新生大鼠肺间歇性缺氧氧化损伤的比较

新生儿间歇性缺氧 (IH) 会导致抗氧化系统不成熟的早产儿氧化窘迫,从而导致肺损伤。辅酶 Q10 (CoQ10) 和鱼油可防止氧化损伤。我们测试了 CoQ10 比鱼油更有效预防新生大鼠 IH 诱导的肺损伤的假设。新生大鼠在出生时暴露于两种临床相关的 IH 范式 (P0):(1) 50% O2 和短暂缺氧 (12% O2);或 (2) 室内空气 (RA) 短暂缺氧 (12% O2),直到 P14,在此期间,他们从 P0 到 P14 每天补充口服 CoQ10、鱼油或橄榄油。幼崽在 P14 时进行研究或放置在 RA 中直到 P21,没有进一步治疗。评估肺的组织病理学和形态测量学;氧化应激和脂质过氧化的生物标志物;和抗氧化剂。在两种新生儿 IH 范式中,21%/12% O2 IH 导致最严重的结果,组织病理学和形态测量学证明了这一点。CoQ10 可有效保留肺结构和减少 IH 诱导的氧化应激生物标志物。相比之下,鱼油会导致严重的不良后果,包括肺泡过度简化、出血、次生嵴形成减少和隔膜增厚。这与升高的氧化剂和抗氧化剂活性有关。数据表明,在 IH 发作之间可能需要更高的 FiO2,以减少 IH 的破坏性影响,并为肺部提供必要的喘息机会。补充鱼油的负面结果表明氧化应激诱导的脂质过氧化。CoQ10 可有效保留肺结构和减少 IH 诱导的氧化应激生物标志物。相比之下,鱼油会导致严重的不良后果,包括肺泡过度简化、出血、次生嵴形成减少和隔膜增厚。这与升高的氧化剂和抗氧化剂活性有关。数据表明,在 IH 发作之间可能需要更高的 FiO2,以减少 IH 的破坏性影响,并为肺部提供必要的喘息机会。补充鱼油的负面结果表明氧化应激诱导的脂质过氧化。CoQ10 可有效保留肺结构和减少 IH 诱导的氧化应激生物标志物。相比之下,鱼油会导致严重的不良后果,包括肺泡过度简化、出血、次生嵴形成减少和隔膜增厚。这与升高的氧化剂和抗氧化剂活性有关。数据表明,在 IH 发作之间可能需要更高的 FiO2,以减少 IH 的破坏性影响,并为肺部提供必要的喘息机会。补充鱼油的负面结果表明氧化应激诱导的脂质过氧化。这与升高的氧化剂和抗氧化剂活性有关。数据表明,在 IH 发作之间可能需要更高的 FiO2,以减少 IH 的破坏性影响,并为肺部提供必要的喘息机会。补充鱼油的负面结果表明氧化应激诱导的脂质过氧化。这与升高的氧化剂和抗氧化剂活性有关。数据表明,在 IH 发作之间可能需要更高的 FiO2,以减少 IH 的破坏性影响,并为肺部提供必要的喘息机会。补充鱼油的负面结果表明氧化应激诱导的脂质过氧化。
更新日期:2021-07-05
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