The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role in biomass degradation, nutrient recycling and ecosystem functioning. Therefore, the use of microbes is an attractive way to transform biomass to produce clean energy and high-value compounds. The microbial degradation of lignocelluloses is a complex process which is dependent upon multiple secreted enzymes and their synergistic activities. The availability of the cutting edge proteomics and highly sensitive mass spectrometry tools make possible for researchers to probe the secretome of microbes and microbial consortia grown on different lignocelluloses for the identification of hydrolytic enzymes of industrial interest and their substrate-dependent expression. This review summarizes the role of secretomics in identifying enzymes involved in lignocelluloses deconstruction, the development of enzyme cocktails and the construction of synthetic microbial consortia for biomass valorization, providing our perspectives to address the current challenges.

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通过分泌组学利用微生物财富促进木质纤维素生物质的价值化：综述

木质纤维素生物质的顽固性是其在工业规模上高价值使用的主要制约因素。在自然界中，微生物在生物质降解、养分循环和生态系统功能方面发挥着至关重要的作用。因此，利用微生物是一种将生物质转化为清洁能源和高价值化合物的有吸引力的方法。木质纤维素的微生物降解是一个复杂的过程，它依赖于多种分泌酶及其协同活性。尖端蛋白质组学和高灵敏度质谱工具的可用性使研究人员能够探测在不同木质纤维素上生长的微生物和微生物群落的分泌组，以鉴定具有工业价值的水解酶及其底物依赖性表达。