Peanut allergy is a major cause of food-induced anaphylaxis and subsequent fatality. Food allergies are becoming an increasingly important global health issue. This study was undertaken to verify the effects of peanut sprout extract (PNSE) on the inhibition of allergic and anaphylactic responses using a peanut (PN)-immunized food allergy mouse model. Fresh peanut sprouts were germinated for 0, 3, 5, and 7 days using mature peanuts. Mice were then sensitized to cholera toxin plus PNE or PNSE by intragastric administration on days 0 and 7, and were then challenged with PNE or PNSE on days 21 and 35. After 5 weeks, we examined the mucosal mast cell degranulation, ear swelling, and systemic anaphylaxis stimulated by PNE extract, in comparison with PNSE. Subsequently, Ara h1, a biomarker of PNE allergy; serum levels; and Th1/Th2 cytokine production in supernatants of cultured splenocytes were measured. PNSE treatment significantly attenuated the secretion of anti-Ara h1 antibody, mucosal mast cell degranulation, degree of systemic anaphylaxis, and ear swelling and increased the production of IFN-γ and IL-10, with a decrease in IL-4 secretion. The results of this study show that the allergenicity of PNE could be reduced by germination, which caused downregulation of Th2 lymphocyte activity, systemic anaphylactic response, and mast cell-mediated ear swelling in PNE-sensitized mice.

花生过敏是食物引起的过敏反应和随后死亡的主要原因。食物过敏正成为日益重要的全球健康问题。本研究旨在验证花生芽提取物 (PNSE) 对使用花生 (PN) 免疫食物过敏小鼠模型抑制过敏和过敏反应的影响。新鲜花生芽使用成熟花生发芽 0、3、5 和 7 天。然后在第 0 天和第 7 天通过胃内给药使小鼠对霍乱毒素加 PNE 或 PNSE 敏感，然后在第 21 天和第 35 天用 PNE 或 PNSE 进行攻击。 5 周后，我们检查了粘膜肥大细胞脱颗粒、耳肿胀和与 PNSE 相比，PNE 提取物刺激的全身性过敏反应。随后，Ara h1，一种PNE过敏的生物标志物；血清水平; 测定培养的脾细胞上清液中的 Th1/Th2 细胞因子产量。PNSE 治疗显着减弱了抗 Ara h1 抗体的分泌、粘膜肥大细胞脱颗粒、全身性过敏反应的程度和耳肿胀，并增加了 IFN-γ 和 IL-10 的产生，同时减少了 IL-4 的分泌。这项研究的结果表明，萌发可以降低 PNE 的过敏性，这会导致 PNE 致敏小鼠的 Th2 淋巴细胞活性下调、全身过敏反应和肥大细胞介导的耳肿胀。IL-4 分泌减少。这项研究的结果表明，萌发可以降低 PNE 的过敏性，这会导致 PNE 致敏小鼠的 Th2 淋巴细胞活性下调、全身过敏反应和肥大细胞介导的耳肿胀。IL-4 分泌减少。这项研究的结果表明，萌发可以降低 PNE 的过敏性，这会导致 PNE 致敏小鼠的 Th2 淋巴细胞活性下调、全身过敏反应和肥大细胞介导的耳肿胀。