The C5 protein encoded by Ageratum leaf curl Sichuan virus is a virulence factor and contributes to the virus infection,Molecular Plant Pathology

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The C5 protein encoded by Ageratum leaf curl Sichuan virus is a virulence factor and contributes to the virus infection
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-07-05 , DOI: 10.1111/mpp.13103
Pengbai Li ₁ , Feng Su ₁ , Qiyuan Meng ₁ , Huabin Yu ₁ , Gentu Wu ₁ , Mingjun Li ₁ , Ling Qing ₁

Affiliation

Earlier reports have indicated that begomoviruses encode four proteins (AC1/C1, AC2/C2, AC3/C3, and AC4/C4 proteins) using complementary-sense DNA as the template. In recent years, several reports have shown that some begomoviruses also encode an AC5/C5 protein from the complementary DNA strand, and these AC5/C5 proteins play different roles in virus infections. Here, we provide evidence showing that Ageratum leaf curl Sichuan virus (ALCScV), a monopartite begomovirus, also encodes a C5 protein that is important for disease symptom formation and can affect viral replication. Infection of Nicotiana benthamiana plants with a potato virus X (PVX)-based vector carrying the ALCScV C5 gene resulted in more severe disease symptoms and higher virus accumulation levels. ALCScV C5 protein can be found in the cytoplasm and the nucleus. Furthermore, this protein is also a suppressor of posttranscriptional gene silencing. Mutational analysis showed that knockout of C5 gene expression significantly reduced ALCScV-induced disease symptoms and virus accumulation, while expression of the C5 gene using the PVX-based vector enhanced ALCScV accumulation in coinfected N. benthamiana plants.

中文翻译：

四川曲叶病毒编码的C5蛋白是一种毒力因子，有助于病毒感染

早期报道表明，begomoviruses 使用互补正义 DNA 作为模板编码四种蛋白质（AC1/C1、AC2/C2、AC3/C3 和 AC4/C4 蛋白质）。近年来，一些报道表明，一些begomoviruses还编码来自互补DNA链的AC5/C5蛋白，这些AC5/C5蛋白在病毒感染中发挥着不同的作用。在此，我们提供的证据表明，藿香叶卷叶四川病毒 (ALCScV)（一种单部分 Begomovirus）也编码一种 C5 蛋白，该蛋白对于疾病症状的形成很重要，并且可以影响病毒复制。用携带 ALCScV C5基因的马铃薯病毒 X (PVX) 载体感染本塞姆氏烟草植物会导致更严重的疾病症状和更高的病毒积累水平。 ALCScV C5 蛋白存在于细胞质和细胞核中。此外，该蛋白也是转录后基因沉默的抑制因子。突变分析表明， C5基因表达的敲除显着减少了 ALCScV 诱导的疾病症状和病毒积累，而使用基于 PVX 的载体表达C5基因则增强了共感染N中 ALCScV 的积累。本塞米亚纳植物。

更新日期：2021-08-12

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