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The association between Hfq and RNase E in long-term nitrogen-starved Escherichia coli
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-07-04 , DOI: 10.1111/mmi.14782
Josh McQuail 1 , Agamemnon J Carpousis 2 , Sivaramesh Wigneshweraraj 1
Affiliation  

Under conditions of nutrient adversity, bacteria adjust metabolism to minimize cellular energy usage. This is often achieved by controlling the synthesis and degradation of RNA. In Escherichia coli, RNase E is the central enzyme involved in RNA degradation and serves as a scaffold for the assembly of the multiprotein complex known as the RNA degradosome. The activity of RNase E against specific mRNAs can also be regulated by the action of small RNAs (sRNA). In this case, the ubiquitous bacterial chaperone Hfq bound to sRNAs can interact with the RNA degradosome for the sRNA guided degradation of target RNAs. The RNA degradosome and Hfq have never been visualized together in live bacteria. We now show that in long-term nitrogen starved E. coli, both RNase E and Hfq co-localize in a single, large focus. This subcellular assembly, which we refer to as the H-body, forms by a liquid-liquid phase separation type mechanism and includes components of the RNA degradosome, namely, the helicase RhlB and the exoribonuclease polynucleotide phosphorylase. The results support the existence of a hitherto unreported subcellular compartmentalization of a process(s) associated with RNA management in stressed bacteria.

中文翻译:

长期缺氮大肠杆菌中 Hfq 与 RNase E 的关联

在营养逆境的情况下,细菌会调整新陈代谢以最大限度地减少细胞能量的消耗。这通常通过控制 RNA 的合成和降解来实现。在大肠杆菌中,RNase E 是参与 RNA 降解的中心酶,并用作组装称为 RNA 降解体的多蛋白复合物的支架。RNase E 针对特定 mRNA 的活性也可以通过小 RNA (sRNA) 的作用来调节。在这种情况下,与 sRNA 结合的普遍存在的细菌伴侣 Hfq 可以与 RNA 降解体相互作用,用于 sRNA 引导的靶 RNA 降解。RNA 降解体和 Hfq 从未在活细菌中一起出现过。我们现在表明,在长期缺氮的大肠杆菌中,RNase E 和 Hfq 共同定位在一个单一的大焦点上。这种亚细胞组装,我们称之为 H 体,通过液-液相分离型机制形成,包括 RNA 降解体的成分,即解旋酶 Rhlb 和外切核糖核酸酶多核苷酸磷酸化酶。结果支持存在迄今为止未报道的与受胁迫细菌中的RNA管理相关的过程的亚细胞区室化。
更新日期:2021-07-04
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