Solvatochromic dyes have emerged as a new class of fluorescent probes in the field of lipid membranes due to their ability to identify the lipid organization of biomembranes in live cells by changing the colour of their fluorescence. This type of solvatochromic function is useful for studying the heterogeneous features of biomembranes caused by the uneven distribution of lipids and cholesterols in live cells and related cellular processes. Therefore, a variety of advanced solvatochromic dyes have been rapidly developed over the last decade. To provide an overview of the works recently developed solvatochromic dyes have enabled, we herein present some solvatochromic dyes, with a particular focus on those based on pyrene and Nile red. As these dyes exhibit preferable photophysical properties in terms of fluorescence microscopy applications and unique distribution/localization in cellular compartments, some have already found applications in cell biological and biophysical studies. The goal of this review is to provide information to researchers who have never used solvatochromic dyes or who have not discovered applications of such dyes in biological studies.

中文翻译：

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用于监测活细胞中生物膜脂质顺序的新兴溶剂致变色推拉染料

溶剂致变色染料已成为脂质膜领域的一类新型荧光探针，因为它们能够通过改变荧光颜色来识别活细胞中生物膜的脂质组织。这种类型的溶剂致变色函数可用于研究由活细胞中脂质和胆固醇的不均匀分布以及相关细胞过程引起的生物膜的异质特征。因此，在过去的十年中，各种先进的溶剂致变色染料得到了迅速发展。为了概述最近开发的溶剂致变色染料已启用的工作，我们在此介绍了一些溶剂致变色染料，特别关注那些基于芘和尼罗红的染料。由于这些染料在荧光显微镜应用和细胞隔室中的独特分布/定位方面表现出较好的光物理特性，因此一些染料已经在细胞生物学和生物物理研究中得到应用。本综述的目的是为从未使用过溶剂致变色染料或尚未发现此类染料在生物学研究中的应用的研究人员提供信息。