The aim of this study was to investigate the effects ionised water has on embryonic development using Wistar rat animal model. For that purpose, alkaline and acidic water was prepared with a domestic water ioniser. It was found that the concentrations of Cl–, SO42– ions increased in acidic water, while in alkaline water, Ca2+ concentration decreased and halogenated hydrocarbon concentrations exceeded permitted levels. The animals were given test alkaline and acidic water, as well as tap water as control. After three months, female rats were mated. On the 21st day of gestation, they were euthanized and subjected to Caesarean sections; the number of live and dead fetuses was recorded. The fetuses were examined for external or visceral malformations and skeletal abnormalities. The data showed that embryo death was higher in acidic and alkaline experimental groups in comparison to the control group. The fetuses in both test groups were significantly shorter than in the control group. Long bones of fetal hind and front limbs were shorter in the acidic group in comparison to the control group. Retardation of limb osteogenesis was expressed in the acidic group fetuses. Therefore, in our model, ionised water had a negative effect on the embryonic development.

中文翻译：

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电离（电解）水对大鼠胚胎发育的影响

本研究的目的是使用 Wistar 大鼠动物模型研究离子水对胚胎发育的影响。为此，使用家用水离子发生器制备碱性和酸性水。结果表明，酸性水中Cl-、SO42-离子浓度升高，而碱性水中Ca2+浓度降低，卤代烃浓度超标。动物被给予测试碱性和酸性水，以及作为对照的自来水。三个月后，雌性大鼠交配。怀孕第21天，安乐死，剖腹产；记录了活胎和死胎的数量。检查胎儿的外部或内脏畸形和骨骼异常。数据显示，与对照组相比，酸性和碱性实验组的胚胎死亡率更高。两个试验组的胎儿均明显短于对照组。与对照组相比，酸性组胎儿后肢和前肢的长骨较短。在酸性组胎儿中表现出肢体成骨的延迟。因此，在我们的模型中，离子水对胚胎发育有负面影响。