The minimal inhibitory concentration (MIC) assay uses agar or broth dilution methods to measure, under defined test conditions, the lowest effective concentration of an antimicrobial agent that inhibits visible growth of a bacterium of interest. This assay is used to test the susceptibilities of bacterial isolates and of novel antimicrobial drugs, and is typically done in nutrient-rich laboratory media that have little relevance to in vivo conditions. As an extension to our original protocol on MIC assays (also published in Nature Protocols), here we describe the application of the MIC broth microdilution assay to test antimicrobial susceptibility in conditions that are more physiologically relevant to infections observed in the clinic. Specifically, we describe a platform that can be applied to the preparation of medium that mimics lung and wound exudate or blood conditions for the growth and susceptibility testing of bacteria, including ESKAPE pathogens. This protocol can also be applied to most physiologically relevant liquid medium and aerobic pathogens, and takes 3–4 d to complete.

最小抑菌浓度 (MIC) 测定使用琼脂或肉汤稀释法来测量，在规定的测试条件下，抑制目标细菌可见生长的抗菌剂的最低有效浓度。该测定用于测试细菌分离物和新型抗菌药物的敏感性，通常在与体内条件无关的营养丰富的实验室培养基中进行。作为我们关于 MIC 检测的原始方案的扩展（也发表在Nature Protocols 上)，在这里我们描述了 MIC 肉汤微量稀释测定法在与临床观察到的感染更生理相关的条件下测试抗菌药物敏感性的应用。具体来说，我们描述了一个平台，该平台可用于制备模拟肺和伤口渗出液或血液条件的培养基，用于细菌（包括 ESKAPE 病原体）的生长和敏感性测试。该协议也可应用于大多数生理相关的液体介质和需氧病原体，需要 3-4 天才能完成。