The aim of this study was to examine the effect of bioactive glass (BAG) loading on the ion release, pH changes, and cell response of the experimental pit and fissure sealant containing sol–gel derived 58S BAG. The BAG and silica filler in different proportion were incorporated into a resin matrix with the final filler loading of 50% in all groups. The specimens were immersed in deionized water (pH 5.8) or lactic acid solution (pH 4.0) at different time points (6 h, 1, 7, 14, 30, and 45 days) for ion release and pH changes assessment. Biological properties were evaluated using MTS assay, alkaline phosphatase (ALP) assays, and alizarin red staining. In the deionized water, the BAG50% group pH reaches to 9.3 and in lactic acid solution to 4.31 after 45 days. The BAG filler percentage did not have a significant effect on ion release in the deionized water. However, ion release increased with the pH reduction, particularly in the BAG50% group. The BAG50% group showed less cytotoxicity and higher ALP activity and calcified nodule formation. The experimental pit and fissure sealant with 50% BAG filler loading exhibited an appropriate increase in pH elevation, ion release, and cell proliferation and differentiation. Therefore, it can be concluded that this bioactive sealant has the potential to hinder secondary caries and can be used as a caries-inhibiting material.

本研究的目的是检查生物活性玻璃 (BAG) 负载对含有溶胶-凝胶衍生 58S BAG 的实验窝沟封闭剂的离子释放、pH 变化和细胞反应的影响。将不同比例的 BAG 和二氧化硅填料掺入树脂基体中，所有组中的最终填料填充量为 50%。将样品在不同时间点（6 小时、1、7、14、30 和 45 天）浸入去离子水（pH 5.8）或乳酸溶液（pH 4.0）中，以评估离子释放和 pH 变化。使用 MTS 测定、碱性磷酸酶 (ALP) 测定和茜素红染色评估生物学特性。在去离子水中，BAG50%组的pH值在45天后达到9.3，在乳酸溶液中达到4.31。BAG 填料百分比对去离子水中的离子释放没有显着影响。然而，离子释放随着 pH 值的降低而增加，尤其是在 BAG50% 组中。BAG50% 组显示出较低的细胞毒性和较高的 ALP 活性和钙化结节形成。实验用 50% BAG 填充剂填充的窝沟封闭剂在 pH 升高、离子释放以及细胞增殖和分化方面表现出适当的增加。因此，可以得出结论，这种生物活性密封剂具有阻止继发龋的潜力，可以用作防龋材料。50% BAG 填充剂的实验性窝沟封闭剂在 pH 升高、离子释放和细胞增殖和分化方面表现出适当的增加。因此，可以得出结论，这种生物活性密封剂具有阻止继发龋的潜力，可以用作防龋材料。50% BAG 填充剂的实验性窝沟封闭剂在 pH 升高、离子释放和细胞增殖和分化方面表现出适当的增加。因此，可以得出结论，这种生物活性密封剂具有阻止继发龋的潜力，可以用作防龋材料。