Abstract

A simple and precise RP-HPLC method has been developed and validated for the separation and trace determination of selected food colorant residues, namely, Tartrazine (E102), Sunset Yellow (E110) and Allura Red (E129), in water samples. The colorants were separated in less than five minutes using buffered distilled water as a mobile phase on a superheated hybrid ODS X-Bridge RP-C18 column (150 × 3.5 µm, 4.6 mm i.d.). Van’t Hoff plots were linear for all the tested colorants at different mobile phases and without any significant changes in their retention factors or the transition stage of the stationary phase. The lower limits of detection for E102 and E110 were found to be 0.5 and 0.016 mg/mL, respectively, whereas for E129 it was 0.0004 mg/mL. The proposed method was checked for its accuracy and precision and was applied to determine the selected colorants in soft drinks and environmental water samples with acceptable recoveries in the range from 83.5 ± 1.9 to 114 ± 3%.

中文翻译：

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一种使用过热水分离和痕量测定食品中特定食用色素残留的环保型 RP-HPLC 方法

摘要

已开发并验证了一种简单而精确的 RP-HPLC 方法，用于分离和痕量测定水样中选定的食用色素残留，即柠檬黄 (E102)、日落黄 (E110) 和诱惑红 (E129)。在过热混合型 ODS X-Bridge RP-C18 色谱柱（150 × 3.5 µm，4.6 mm 内径）上，使用缓冲蒸馏水作为流动相，在不到 5 分钟的时间内分离着色剂。Van't Hoff 图对于所有测试的着色剂在不同流动相下都是线性的，并且它们的保留因子或固定相的过渡阶段没有任何显着变化。发现 E102 和 E110 的检测下限分别为 0.5 和 0.016 mg/mL，而 E129 的检测下限为 0.0004 mg/mL。