A new trinuclear Cu(II) complex [Cu₃(μ₃-OH)(μ-pz)₃(Hpz)₂(HCOO)(BTAO)Cl] (1) (Hpz = pyrazole, BTAO = 4,6-bis(dimethylamino)-1,3,5-triazin-2(1H)-one) and four mononuclear complexes [M(Hpz)₄Cl₂] [M²⁺ = Cu²⁺, Co²⁺, Ni²⁺ and Zn²⁺] (2–5) have been synthesized and characterized. Electronic absorption spectra, fluorescence spectra and circular dichroism spectra were carried out to investigate the interactions between complexes 1–5 and CT-DNA. These complexes exhibited different binding affinity with DNA, and the order of DNA binding strength was 1 > 2 > 4 > 3 > 5. Most of these complexes could cleave pBR322 DNA efficiently in the presence of H₂O₂ as an activator, and the cleavage efficacy of these complexes was quite consistent with their DNA binding abilities. Furthermore, the in vitro cytotoxicity studies of complexes 1–5 were measured by MTT assay. The trinuclear Cu(II) complex 1 showed cytotoxicity against human lung carcinoma cell lines A549 (IC₅₀ = 24.314 ± 1.386 µM) and human esophageal carcinoma cell line EC109 (IC₅₀ = 27.335 ± 1.437 µM), while the mononuclear complexes 2–5 did not show apparent cytotoxicity to the tested cell lines (IC₅₀ > 50 μM). The different cytotoxicity of these complexes may also be relevant to their reactivity toward DNA.

一种新的三核 Cu(II) 配合物 [Cu ₃ ( μ ₃ -OH)( μ -pz) ₃ (Hpz) ₂ (HCOO)(BTAO)Cl] ( 1 ) (Hpz = 吡唑，BTAO = 4,6-bis (二甲氨基)-1,3,5-triazin-2(1 H )-one) 和四个单核配合物 [M(Hpz) ₄ Cl ₂ ] [M ²⁺ = Cu ²⁺ , Co ²⁺ , Ni ²⁺和Zn ²⁺ ] ( 2-5 ) 已被合成和表征。进行电子吸收光谱、荧光光谱和圆二色光谱以研究配合物1-5之间的相互作用和 CT-DNA。这些复合物与DNA的结合亲和力不同，DNA结合强度大小为1 > 2 > 4 > 3 > 5。在H ₂ O ₂作为激活剂存在的情况下，这些复合物中的大多数可以有效地切割pBR322 DNA ，并且这些复合物的切割功效与其DNA结合能力非常一致。此外，复合物1-5 的体外细胞毒性研究通过 MTT 测定进行测量。三核 Cu(II) 复合物1对人肺癌细胞系 A549 (IC ₅₀= 24.314 ± 1.386 µM) 和人食管癌细胞系 EC109 (IC ₅₀ = 27.335 ± 1.437 µM)，而单核复合物2–5对测试的细胞系没有表现出明显的细胞毒性 (IC ₅₀ > 50 µM)。这些复合物的不同细胞毒性也可能与它们对 DNA 的反应性有关。