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Phytochemical Composition, Antioxidant, Antiacetylcholinesterase, and Cytotoxic Activities of Rumex crispus L.
International Journal of Analytical Chemistry ( IF 1.5 ) Pub Date : 2021-07-03 , DOI: 10.1155/2021/6675436
Mohamed Marouane Saoudi 1, 2 , Jalloul Bouajila 2 , Rami Rahmani 2, 3 , Khaled Alouani 1
Affiliation  

Rumex crispus L. (R. crispus) is regarded as an aromatic plant. It was used for its excellent biological properties in traditional medicine. The aerial part was extracted successively by maceration with three solvents increasing polarity (cyclohexane (CYH), dichloromethane (DCM), and methanol (MeOH)) to evaluate their chemical compositions and biological activities. The extracts were rich in phenolic compounds (13.0 to 249.8 mg GAE/g of dry weight (dw)). The MeOH extract has presented remarkable IC50 = 6.2 μg/mL for anti-DPPH and 31.6 μg/mL for anti-AChE. However, the DCM extract has the highest cytotoxic activity against the two cancer cells (HCT-116 and MCF-7) (69.2 and 77.2% inhibition at 50 μg/mL, respectively). Interestingly, GC-MS analysis enabled to identify three new compounds in R. crispus extracts, such as L-(−)-arabitol (5), D-(−) fructopyranose (7) detected only in MeOH extract, and 2, 5-dihydroxyacetophenone (3) detected in all extracts. For HPLC chromatograms, cardamonin (8), 5-hydroxy-3′-methoxyflavone (17), and 3′-hydroxy-b-naphthoflavone (18) showed the highest concentrations of 74.0, 55.5, and 50.4 mg/g of dw, respectively, among others who are identified. Some phenolic compounds were identified and quantified by HPLC in more than one organic extract, such as 4′, 5-dihydroxy-7-methoxyflavone (13), 4′, 5-dihydroxy-7-methoxyflavone (14), 5-hydroxy-3′-methoxyflavone (17), and 3′-hydroxy-b-naphthoflavone (18), were found for the first time in the R. crispus extracts. Our results showed that the biological activities of this plant might be linked to their phenolic compounds and that the polar extracts could be considered as new natural supplements to be used in food and pharmaceuticals.

中文翻译:

瘤胃的植物化学成分、抗氧化剂、抗乙酰胆碱酯酶和细胞毒活性。

Rumexcrispus L.(R.crispus)被认为是一种芳香植物。它在传统医学中因其优异的生物学特性而被使用。通过用三种极性增加的溶剂(环己烷(CYH)、二氯甲烷(DCM)和甲醇(MeOH))浸渍连续提取地上部分,以评估它们的化学成分和生物活性。提取物富含酚类化合物(13.0 至 249.8 mg GAE/g 干重 (dw))。MeOH 提取物具有显着的 IC 50  = 6.2  μ g/mL 抗 DPPH 和 31.6  μ g/mL 抗 AChE。然而,DCM 提取物对两种癌细胞(HCT-116 和 MCF-7)具有最高的细胞毒活性(在 50 μ 时抑制 69.2% 和 77.2% 克/毫升,分别)。有趣的是,GC-MS 分析能够鉴定出卷曲草提取物中的三种新化合物,例如 L-(-)-阿拉伯糖醇 (5)、仅在甲醇提取物中检测到的 D-(-) 吡喃果糖 (7),以及 2, 5 -二羟基苯乙酮 (3) 在所有提取物中检测到。对于 HPLC 色谱图,小豆蔻素 (8)、5-羟基-3'-甲氧基黄酮 (17) 和 3'-羟基-b-萘黄酮 (18) 显示最高浓度为 74.0、55.5 和 50.4 mg/g dw,分别,其中包括哪些人。一些酚类化合物通过 HPLC 在一种以上的有机提取物中得到鉴定和定量,例如 4', 5-dihydroxy-7-methoxyflavone (13), 4', 5-dihydroxy-7-methoxyflavone (14), 5-hydroxy- 3'-methoxyflavone(17),和3'-羟基-b-萘黄酮(18)中,发现在第一次R.草提取物。我们的研究结果表明,这种植物的生物活性可能与其酚类化合物有关,并且极性提取物可被视为用于食品和药物的新型天然补充剂。
更新日期:2021-07-04
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