Biomass Conversion and Biorefinery ( IF 3.5 ) Pub Date : 2021-07-03 , DOI: 10.1007/s13399-021-01642-3 Jianming Yu 1 , Zhaoxian Xu 1 , Sitong Chen 1 , Shengwei Wang 1 , Yang Yu 1 , Mingjie Jin 1 , Hao He 2
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Xylose fermentation is an essential issue for lignocellulosic ethanol production, which is often inhibited by degradation products generated during biomass pretreatment. In this study, enzymatic hydrolysate of dilute acid pretreated corn stover was fermented using Zymomonas mobilis 8b. With the solid loading of corn stover increased from 5 to 24 wt.%, the xylose consumption volume decreased from 97.0 to 21.5%. The ethanol produced ranged from 7.8 to 28.6 g/L. In order to improve xylose consumption, corn slurry was integrated into the corn stover ethanol process. Integration strategies, glucoamylase dosage, and corn–corn stover mixing ratio were investigated. Results showed that introducing corn slurry into corn stover hydrolysate was an effective way to improve xylose fermentation as well as enhance ethanol titer. Specifically, when liquefied corn slurry was introduced into corn stover hydrolysate at the ratio of 1:1 and total solid loading of 24%, the xylose consumption volume exceeded 88% with the final ethanol titer reached 68.6 g/L. This study indicates that integration of the first- and the second-generation ethanol processes is beneficial to xylose fermentation.
Graphical abstract
中文翻译：
整合玉米乙醇和玉米秸秆乙醇工艺以提高木糖发酵性能
木糖发酵是木质纤维素乙醇生产的一个重要问题，其通常受到生物质预处理过程中产生的降解产物的抑制。在这项研究中，稀酸预处理玉米秸秆的酶促水解物使用运动发酵单胞菌发酵8b. 随着玉米秸秆固含量从5wt.%增加到24wt.%，木糖消耗量从97.0%下降到21.5%。产生的乙醇范围为 7.8 至 28.6 g/L。为了提高木糖消耗，玉米浆被整合到玉米秸秆乙醇工艺中。研究了整合策略、葡糖淀粉酶剂量和玉米-玉米秸秆混合比例。结果表明，将玉米浆加入玉米秸秆水解液中是改善木糖发酵和提高乙醇效价的有效途径。具体地，当液化玉米浆以1:1的比例和24%的总固体负载量加入玉米秸秆水解液中时，木糖消耗量超过88%，最终乙醇效价达到68.6 g/L。
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