Many models assessing the risk of sepsis utilize the knowledge of the constituents of the plasminogen system, as it is proven that some species of bacteria can activate plasminogen, as a result of interactions with bacterial outer membrane proteins. However, much is yet to be discovered about this interaction since there is little information regarding some bacterial species. This study is aimed to check if Klebsiella pneumoniae, one of the major factors of nosocomial pneumonia and a factor for severe sepsis, has the ability to bind to human plasminogen. The strain used in this study, PCM 2713, acted as a typical representative of the species. With use of various methods, including: electron microscopy, 2-dimensional electrophoresis, immunoblotting and peptide fragmentation fingerprinting, it is shown that Klebsiella pneumoniae binds to human plasminogen, among others, due to plasminogen-bacterial enolase-like protein interaction, occurring on the outer membrane of the bacterium. Moreover, the study reveals, that other proteins, such as: phosphoglucomutase, and phosphoenolpyruvate carboxykinase act as putative plasminogen-binding factors. These information may virtually act as a foundation for future studies investigating: the: pathogenicity of Klebsiella pneumoniae and means for prevention from the outcomes of Klebsiella-derived sepsis.

许多评估脓毒症风险的模型利用了纤溶酶原系统成分的知识，因为已证明某些细菌种类可以激活纤溶酶原，这是与细菌外膜蛋白相互作用的结果。然而，关于这种相互作用还有很多待发现，因为关于某些细菌种类的信息很少。本研究旨在检查肺炎克雷伯菌（医院获得性肺炎的主要因素之一和严重脓毒症的因素之一）是否具有与人纤溶酶原结合的能力。本研究中使用的菌株 PCM 2713 是该物种的典型代表。使用各种方法，包括：电子显微镜、二维电泳、免疫印迹和肽片段指纹图谱，表明由于纤溶酶原-细菌烯醇化酶样蛋白相互作用，肺炎克雷伯菌与人纤溶酶原结合，发生在细菌的外膜上。此外，该研究表明，其他蛋白质，例如：磷酸葡萄糖变位酶和磷酸烯醇丙酮酸羧激酶，可作为推定的纤溶酶原结合因子。这些信息实际上可以作为未来研究调查的基础：肺炎克雷伯菌的致病性和预防克雷伯菌衍生败血症结果的方法。