Stress adversely affects the cellular and electrophysiological mechanisms of memory; however, crocin has beneficial effects on brain functions. Nonetheless, the electrophysiological effects of using this active saffron component at different doses are not yet studied in rats under chronic restraint stress. Therefore, this study compared the impact of crocin at different doses on the excitability and long-term potentiation (LTP) in the CA1 area of rats, as well as their electroencephalogram (EEG) responses, hippocampal and frontal cortical glucose levels under chronic restraint stress (an emotional stress model). Forty rats were allocated into five groups of control, sham, restraint stress (6 h/day/21 days), and two stress groups receiving intraperitoneal injections of crocin (30, 60 mg/kg/day). Besides measuring the slope and amplitude of field excitatory postsynaptic potentials (fEPSPs) in the input–output and LTP curves, the EEG waves and hippocampal and frontal cortical glucose levels were assessed in all groups. Chronic restraint stress significantly decreased the fEPSP slope and amplitude in the input–output curves and after LTP induction. Both doses of crocin (60 and particularly 30 mg/kg) significantly improved fEPSP slope and amplitude in the stressed groups. Also, stress and crocin only at a dose of 30 mg/kg altered the EEG waves. Hippocampal and frontal cortical glucose levels displayed no significant differences in the experimental groups. Crocin at doses of 60 mg/kg/day and particularly 30 mg/kg/day reversed the harmful effects of chronic restraint stress on LTP as a cellular memory-related mechanism. However, only the lower dose of crocin affected the electrical brain activity in EEG.

压力会对记忆的细胞和电生理机制产生不利影响；然而，藏红花素对大脑功能有益。尽管如此，尚未在慢性约束压力下的大鼠中研究以不同剂量使用这种活性藏红花成分的电生理效应。因此，本研究比较了不同剂量藏红花素对大鼠CA1区兴奋性和长时程增强（LTP）的影响，以及慢性约束应激下大鼠脑电图（EEG）反应、海马和额叶皮质葡萄糖水平的影响。 （情绪压力模型）。将 40 只大鼠分为 5 个对照组、假手术组、约束应激组（6 小时/天/21 天）和两个接受腹腔注射藏红花素（30、60 mg/kg/天）的应激组。除了测量输入-输出和 LTP 曲线中场兴奋性突触后电位 (fEPSP) 的斜率和幅度外，还评估了所有组的 EEG 波和海马和额叶皮质葡萄糖水平。慢性约束应力显着降低了输入-输出曲线和 LTP 诱导后的 fEPSP 斜率和幅度。两种剂量的藏红花素（60 和特别是 30 mg/kg）都显着改善了压力组的 fEPSP 斜率和幅度。此外，仅 30 mg/kg 剂量的压力和藏红花素改变了 EEG 波。海马和额叶皮质葡萄糖水平在实验组中没有显示出显着差异。剂量为 60 mg/kg/天，特别是 30 mg/kg/天的藏红花素可逆转慢性束缚应激对 LTP 作为细胞记忆相关机制的有害影响。然而，