Peptide nucleic acids (PNAs) have primarily been used to achieve therapeutic gene modulation through antisense strategies since their design in the 1990s. However, the application of PNAs as a functional nanomaterial has been more recent. We recently reported that γ-modified peptide nucleic acids (γPNAs) could be used to enable formation of complex, self-assembling nanofibers in select polar aprotic organic solvent mixtures. Here we demonstrate that distinct γPNA strands, each with a high density of γ-modifications can form complex nanostructures at constant temperatures within 30 minutes. Additionally, we demonstrate DNA-assisted isothermal growth of γPNA nanofibers, thereby overcoming a key hurdle for future scale-up of applications related to nanofiber growth and micropatterning.

中文翻译：

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γPNA纳米纤维在恒温下的快速自组装

自 1990 年代设计以来，肽核酸 (PNA) 主要用于通过反义策略实现治疗性基因调节。然而，PNA 作为功能性纳米材料的应用是最近才出现的。我们最近报道了γ修饰的肽核酸 ( γ PNA) 可用于在选择的极性非质子有机溶剂混合物中形成复杂的自组装纳米纤维。在这里，我们证明了不同的γ PNA 链，每个链都具有高密度的γ修饰，可以在 30 分钟内在恒温下形成复杂的纳米结构。此外，我们展示了γ的 DNA 辅助等温生长PNA 纳米纤维，从而克服了未来扩大与纳米纤维生长和微图案相关应用的关键障碍。