The determination of enantio-separation, absolute configuration and chiral recognition mechanism of ofloxacin and flumequine was carried out by high-performance liquid chromatography (HPLC) and modeling studies. The column used was a Chiralcel OD-H with normal and polar organic mobile phase modes. The exhaustive chiral separation was optimized through various HPLC parameters.

中文翻译：

---

高效液相色谱法和建模研究测定氧氟沙星和氟甲喹的对映体分离、绝对构型和手性识别机制

氧氟沙星和氟甲喹的对映体分离、绝对构型和手性识别机制的测定通过高效液相色谱(HPLC)和建模研究进行。使用的色谱柱是具有正常和极性有机流动相模式的 Chiralcel OD-H。通过各种 HPLC 参数优化了彻底的手性分离。