Pod and seed weight per plant (PSW) is one of the most important components of soybean yield. We analysed 147 recombinant inbred lines (RILs) of soybean. Using a combination of simple sequence repeat (SSR) and specific-length amplified fragment (SLAF) high-density maps, we applied composite interval mapping (CIM) and multiple interval mapping (MIM) to map quantitative trait loci (QTLs) for PSW across multiple years (2006–10 and 2013). We mapped 24 QTLs for PSW, with 10 QTLs identified by SSR genetic map and 14 by SLAF genetic map. Five consensus QTLs were integrated, and they were validated by a chromosome segment substitution line (CSSL) population. Furthermore, the functions of all genes located in consensus QTL intervals were predicted; nine candidate genes function directly or indirectly in regulating seed development as well as seed size and weight. Our results lay a foundation for the cloning of candidate genes related to PSW and marker-assisted breeding in soybean.

每株豆荚和种子重量 (PSW) 是大豆产量的最重要组成部分之一。我们分析了 147 个大豆重组自交系 (RIL)。使用简单序列重复 (SSR) 和特定长度扩增片段 (SLAF) 高密度图的组合，我们应用复合区间作图 (CIM) 和多区间作图 (MIM) 来绘制 PSW 的数量性状基因座 (QTL)。多年（2006-10 和 2013）。我们为 PSW 绘制了 24 个 QTL，其中 10 个 QTL 通过 SSR 遗传图谱确定，14 个通过 SLAF 遗传图谱确定。整合了五个共有 QTL，并通过染色体片段置换系 (CSSL) 群体进行验证。此外，预测了位于共有 QTL 区间的所有基因的功能；九个候选基因直接或间接地调节种子发育以及种子大小和重量。我们的研究结果为大豆PSW相关候选基因的克隆和标记辅助育种奠定了基础。