Plant Foods for Human Nutrition ( IF 3.1 ) Pub Date : 2021-07-03 , DOI: 10.1007/s11130-020-00873-5 Olha Mykhailenko 1 , Ivan Bezruk 1 , Liudas Ivanauskas 2 , Victoriya Georgiyants 1
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Crocus sativus L. is one of the most valuable crops, which stigmas are used as the food supplement and herbal medicine. In Ukraine, the cultivation of C. sativus began in 2015, and in this regard, it became possible to conduct a comparative content analysis of the major metabolites (crocin, picrocrocin, safranal) using the validated HPLC method in the stigmas from the different country regions. The grinding technique of Crocus stigmas in liquid nitrogen was used for the first time, which influenced an increase in the yield of trans-crocetin bis(β-D-gentiobiosyl) ester in 3.5 times compared with a normal grinding. Samples from the northeastern regions of cultivation such as Zaporizhia and Chernigiv had the higher amount of crocin (205 and 226 mg/g, respectively), while in the samples of the southwestern region the decrease of the amount of crocin was observed (180 mg/g and less), which was due to the climatic features of the country. These results can be utilized in the pharmaceutical and food industries for creating food additives for human nutrition.
Graphical Abstract
中文翻译：
乌克兰藏红花样品主要代谢物的 HPLC 比较分析
番红花是最有价值的作物之一，其柱头被用作食品补充剂和草药。在乌克兰，C. sativus的栽培始于 2015 年，在这方面，可以使用经验证的 HPLC 方法对来自不同国家的柱头进行主要代谢物（藏红花苷、苦藏红素、番红花醛）的含量比较分析地区。首次采用液氮研磨番红花工艺，影响了反式番红花素双( β)收率的提高。-D-gentiobiosyl) 酯是正常研磨的 3.5 倍。扎波罗热和切尔尼格夫等东北地区的样品中藏红花素含量较高（分别为 205 和 226 毫克/克），而在西南地区的样品中，藏红素含量下降（180 毫克/克）。 g 和更少），这是由于该国的气候特征。这些结果可用于制药和食品工业，用于为人类营养创造食品添加剂。
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