Studies have reported that the incidence of ocular discomfort in people who often wear makeup is higher than that in the normal population. The incidence of ocular discomfort of these people may be also related to the daily ocular exposure to chemical surfactants during cleaning. The objectives of this study were to explore morphological and pathological changes in the murine ocular surface after low-dose repeated exposure to disodium cocoamphodiacetate (DC), a kind of chemical surfactant widely used in personal cleaning products, and to investigate the possible mechanisms. DC was administered in low dose (0.1%) to the ocular surface of C56BL/6 once daily for two weeks. We found that there were an increase of sodium fluorescein staining on the cornea, a significant thinning of corneal epithelial thickness, and increased TUNEL-positive cells in corneal epithelium in vivo. DC treatment also modulated the distribution of K14⁺ and P63⁺ epithelia from the limbal to the center on the cornea. In cultured murine corneal epithelial progenitor cell line (TKE2), DC treatment induced cell detachment and decreased the activation of Ak strain transforming protein (AKT), and extracellular signal-regulated kinase (ERK). And DC increased TUNEL-positive cells in vitro with increased expression of cleaved Caspase3 and B-cell lymphoma-2 associated X protein (Bax). Our results indicated that repeated low-dose DC exposure on ocular surface caused significant impairment on the structure and viability of the corneal epithelium by inhibiting epithelial proliferation and inducing apoptosis. It provides the foundations to understand the harmful effects of cleaning products daily exposure on the ocular surface.

研究报告称，经常化妆的人眼部不适的发生率高于正常人群。这些人眼部不适的发生率也可能与日常清洁时眼部接触化学表面活性剂有关。本研究的目的是探讨低剂量反复接触椰油两性二乙酸二钠 (DC)（一种广泛用于个人清洁产品的化学表面活性剂）后小鼠眼表的形态学和病理学变化，并探讨其可能的机制。DC 以低剂量 (0.1%) 施用于 C56BL/6 的眼表，每天一次，持续两周。我们发现角膜上荧光素钠染色增加，角膜上皮厚度显着变薄，并在体内增加角膜上皮中的 TUNEL 阳性细胞。直流处理也调节了 K14 的分布⁺和 P63 ⁺上皮细胞从角膜缘到角膜中央。在培养的小鼠角膜上皮祖细胞系 (TKE2) 中，DC 处理诱导细胞脱离并降低 Ak 菌株转化蛋白 (AKT) 和细胞外信号调节激酶 (ERK) 的活化。DC 在体外增加了 TUNEL 阳性细胞，同时增加了裂解的 Caspase3 和 B 细胞淋巴瘤-2 相关 X 蛋白 (Bax) 的表达。我们的结果表明，眼表反复低剂量 DC 暴露通过抑制上皮增殖和诱导细胞凋亡，对角膜上皮的结构和活力造成显着损害。它为了解清洁产品日常接触对眼表的有害影响奠定了基础。