Abstract

Liquid wastes (LW) disposed in hospital handwashing sinks may affect colonization of sink P-traps by carbapenemase-producing Klebsiella pneumoniae (CPKP), causing CPKP dispersal into the patient care environment. This study aimed to determine the effect of LW on biofilm formation and CPKP colonization in a P-Trap model (PTM). PTMs containing polymicrobial biofilms grown in autoclaved municipal tap water (ATW) supplemented with 5% dextrose in water (D5W), nutritional shake (Shake), sugar-based soft drink (Soda), or ATW were inoculated with K. pneumoniae ST258 KPC+ (ST258) or K. pneumoniae CAV1016 (CAV1016) and sampled after 7, 14, and 21 d. Biofilm bio-volume, mean thickness, and heterotrophic plate counts were significantly reduced and roughness coefficient significantly increased by Soda compared with D5W, Shake, or ATW. CPKP were significantly reduced by Soda but significantly amplified by D5W (ST258; CAV1016, 7 d) and Shake (ST258) suggesting that reducing LW disposal in sinks may reduce CPKP dispersal into patient care environments.

摘要

处置在医院洗手池中的液体废物 (LW) 可能会影响产生碳青霉烯酶的肺炎克雷伯菌(CPKP)对水槽 P 陷阱的定植，导致 CPKP 扩散到患者护理环境中。本研究旨在确定 LW 对 P-Trap 模型 (PTM) 中生物膜形成和 CPKP 定植的影响。含有在高压灭菌的市政自来水 (ATW) 中生长的多微生物生物膜的 PTM，在水中添加 5% 葡萄糖 (D5W)、营养奶昔 (Shake)、糖基软饮料 (Soda) 或 ATW，并接种肺炎克雷伯菌ST258 KPC+。 ST258) 或肺炎克雷伯菌CAV1016 (CAV1016) 并在 7、14 和 21 天后采样。与 D5W、Shake 或 ATW 相比，Soda 的生物膜生物体积、平均厚度和异养平板计数显着降低，粗糙度系数显着增加。CPKP 被 Soda 显着降低，但被 D5W (ST258; CAV1016, 7 d) 和 Shake (ST258) 显着放大，表明减少水槽中的 LW 处理可能会减少 CPKP 扩散到患者护理环境中。