Evaluation of the protective effect on enamel demineralization of CPP-ACP paste and ROCS by vibrational spectroscopy and SAXS: An in vitro study,Microscopy Research and Technique

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Evaluation of the protective effect on enamel demineralization of CPP-ACP paste and ROCS by vibrational spectroscopy and SAXS: An in vitro study
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-07-02 , DOI: 10.1002/jemt.23857
Ozlem Marti Akgun ₁ , Sevgi Haman Bayari ₂ , Semra Ide ₂ , Gunseli Guven Polat ₃ , Ceren Yildirim ₄ , Ilgar Orujalipoor ₄

Affiliation

The aim of this study was to investigate human dental enamel surfaces using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), Raman spectroscopy, and small angle X-ray scattering (SAXS) techniques concerning differences between the demineralized enamel surface and remineralized enamel surface by casein phosphopeptide amorphous calcium phosphate, Tooth mousse® (CPP-ACP) and remineralizing oral care systems (ROCS®) agents within the same tooth. For this purpose, 20 freshly extracted human maxillary central incisors without caries and defects were used. Labial surfaces of each of the teeth were divided into four sections, which were marked as follows: Group 1, normal enamel; Group 2, demineralized enamel with demineralization solution; Group 3, demineralized enamel + remineralization agent (ROCS for 10 teeth, CPP-ACP for 10 teeth); and Group 4, remineralization agent (ROCS for 10 teeth, CPP-ACP for 10 teeth). To describe the changes in tooth enamel, the phosphate group concentration within enamel was used as an indicator of the degree of mineralization. The phosphate and carbonate bands in the FTIR and Raman spectra were used to investigate the structural changes in the demineralized and remineralized enamel. Spectroscopic data were statistically analyzed in terms of CPP-ACP and ROCS using one-way analysis of variance. The carbonate content of demineralized enamel was higher than the carbonate content in the other groups (p < .03). The apatite carbonate-phosphate balance in the samples with only remineralizing agent—especially ROCS applied—changed significantly (p < .05) compared to the normal group. The average FTIR spectra of the groups were subjected to multivariate hierarchical cluster analysis (HCA) conducted with the use of the OPUS 5.5 software. Nanosized surface morphologies of the samples were compared using pair distance distributions obtained through SAXS analyses. According to the SAXS analyses, applications of CCP + ACP and ROCS agents were effective on nanostructures for all groups.

中文翻译：

通过振动光谱和 SAXS 评估 CPP-ACP 糊剂和 ROCS 对牙釉质脱矿的保护作用：一项体外研究

本研究的目的是使用衰减全反射傅里叶变换红外光谱 (ATR-FTIR)、拉曼光谱和小角 X 射线散射 (SAXS) 技术研究人类牙釉质表面，研究脱矿牙釉质表面和再矿化牙釉质之间的差异。通过酪蛋白磷酸肽无定形磷酸钙、Tooth Mousse® (CPP-ACP) 和再矿化口腔护理系统 (ROCS®) 药剂在同一颗牙齿内表面。为此，使用了 20 颗新鲜拔出的无龋齿和缺损的人上颌中切牙。每颗牙齿的唇面分为四个部分，标记如下：组1，正常牙釉质；第 2 组，用脱矿溶液脱矿牙釉质；第 3 组，脱矿牙釉质 + 再矿化剂（10 颗牙齿的 ROCS，10颗牙齿的CPP-ACP）；第4组，再矿化剂（10颗牙的ROCS，10颗牙的CPP-ACP）。为了描述牙釉质的变化，牙釉质内的磷酸基团浓度被用作矿化程度的指标。FTIR 和拉曼光谱中的磷酸盐和碳酸盐谱带用于研究脱矿和再矿化牙釉质的结构变化。使用单向方差分析根据 CPP-ACP 和 ROCS 对光谱数据进行统计分析。脱矿牙釉质的碳酸盐含量高于其他组的碳酸盐含量（牙釉质内的磷酸基团浓度被用作矿化程度的指标。FTIR 和拉曼光谱中的磷酸盐和碳酸盐谱带用于研究脱矿和再矿化牙釉质的结构变化。使用单向方差分析根据 CPP-ACP 和 ROCS 对光谱数据进行统计分析。脱矿牙釉质的碳酸盐含量高于其他组的碳酸盐含量（牙釉质内的磷酸基团浓度被用作矿化程度的指标。FTIR 和拉曼光谱中的磷酸盐和碳酸盐谱带用于研究脱矿和再矿化牙釉质的结构变化。使用单向方差分析根据 CPP-ACP 和 ROCS 对光谱数据进行统计分析。脱矿牙釉质的碳酸盐含量高于其他组的碳酸盐含量（p < .03)。与正常组相比，仅使用再矿化剂（尤其是 ROCS）的样品中的磷灰石碳酸盐-磷酸盐平衡发生显着变化 ( p < .05)。使用 OPUS 5.5 软件对各组的平均 FTIR 光谱进行多变量层次聚类分析 (HCA)。使用通过 SAXS 分析获得的对距离分布比较样品的纳米尺寸表面形态。根据 SAXS 分析，应用 CCP + ACP 和 ROCS 试剂对所有组的纳米结构都有效。

更新日期：2021-07-02

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