Using optical spectroscopy, we have studied the models of lipid peroxidation in mitochondrial membranes isolated from the hearts of rats. The oxidative efficiency of tested iron-containing complexes was assessed by the level of TBA-reactive compounds formed in the samples as the main secondary products of peroxidation. EPR spectroscopy of spin labels was used to study the changes in structural and conformational characteristics of mitochondrial membrane lipids in the presence of oxidation initiators. Mitochondrial suspensions were incubated with prooxidants, mostly myoglobin and cumene hydroperoxide, to detect the changes. In this study, we used spin labeled molecules of 5-doxylstearic and 16-doxylstearic acid with paramagnetic NO-fragments attached to different sites of the hydrocarbon chain. With the use of oxidation models, it was possible to obtain preparations of mitochondrial membranes with different degrees of oxidative modification. It was found that the combination of the initiators of oxidation, i.e., cumene hydroperoxide (or tert-butyl hydroperoxide), resulted in the failure of paramagnetiс centers of the spin-labeled membrane.

我们使用光谱学研究了从大鼠心脏中分离出来的线粒体膜中脂质过氧化的模型。测试的含铁配合物的氧化效率通过样品中作为过氧化的主要副产物形成的 TBA 反应性化合物的水平来评估。自旋标记的 EPR 光谱用于研究在氧化引发剂存在下线粒体膜脂的结构和构象特征的变化。线粒体悬浮液与促氧化剂一起孵育，主要是肌红蛋白和枯烯氢过氧化物，以检测变化。在这项研究中，我们使用自旋标记的 5-强力硬脂酸和 16-强力硬脂酸分子，顺磁性 NO 片段连接到烃链的不同位点。使用氧化模型，可以获得不同程度氧化修饰的线粒体膜制剂。发现氧化引发剂，即氢过氧化枯烯（或叔丁基氢过氧化物）的组合导致自旋标记膜的顺磁性中心失效。