The emergence of outbreaks of foodborne illness is closely associated with food contamination caused by various enteric pathogens, such as Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus. The control of enteric pathogens poses a challenge due to the fact that these pathogens can persist for a long period of time in the environment. The rapid detection of pathogenic organisms plays a crucial role in the prevention and identification of crises related to health, safety, and well-being. Improper sample handling and processing may influence the diagnostic efficacy and accuracy. The aim of the present study was to compare the preservation capacity for enteric bacteria between Whatman Flinders Technology Associates (FTA) cards and swabs for reverse transcription-quantitative PCR (RT-qPCR) detection. It was found that Whatman FTA cards exhibited an improved preservation capacity for five types (both laboratory and environmental strains) of enteric bacteria, including Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus for RT-qPCR detection. Hence, Whatman FTA cards may be a suitable tool for the routine isolation of foodborne bacteria for molecular diagnosis. Therefore, the use of Whatman FTA cards for sample collection and preservation may increase sensitivity and accuracy for bacteria isolation and diagnosis.

中文翻译：

---

Whatman FTA Elute Cards 和传统拭子在 RT-qPCR 检测中用于检测肠道致病菌的比较

食源性疾病暴发的出现与各种肠道病原体引起的食物污染密切相关，如大肠杆菌O157:H7、单核细胞增生李斯特菌、肠炎沙门氏菌和金黄色葡萄球菌. 由于这些病原体可以在环境中长期存在，肠道病原体的控制构成了挑战。病原生物的快速检测在预防和识别与健康、安全和福祉相关的危机方面起着至关重要的作用。样本处理和处理不当可能会影响诊断效力和准确性。本研究的目的是比较 Whatman Flinders Technology Associates (FTA) 卡和用于逆转录定量 PCR (RT-qPCR) 检测的拭子对肠道细菌的保存能力。发现 Whatman FTA 卡对五种类型（实验室和环境菌株）肠道细菌的保存能力有所提高，包括大肠杆菌O157:H7、用于 RT-qPCR 检测的单核细胞增生李斯特菌、肠炎沙门氏菌血清型和金黄色葡萄球菌。因此，Whatman FTA 卡可能是用于分子诊断的食源性细菌常规分离的合适工具。因此，使用 Whatman FTA 卡进行样本收集和保存可以提高细菌分离和诊断的灵敏度和准确性。