Organophosphate (OP) compounds comprise one of the most widely used classes of insecticides worldwide. OPs have been shown to have negative human health impacts, particularly developmental neurotoxicity. However, neurotoxic impacts in later adulthood and during the aging process are relatively uncharacterized. The present study examined diazinon (DZN), an OP, to determine the neurobehavioral consequences, in addition to mitochondrial dysfunction on a macroscale (whole organism basal respiration) and on a microscale (whole organ mitochondrial respiration), using zebrafish (ZF) as a model. One group of 14-month-old adult ZF were exposed acutely as adults (0.4, 1.25, and 4.0 μM) for five days and tested as adults, and another group was exposed developmentally 5–120 h post-fertilization (70, 210, and 700 nM) and tested at larval, adolescent, adult, and aging life stages. ZF exposed acutely as adults did not display many significant neurobehavioral impacts or mitochondrial dysfunction. Conversely, the embryonically exposed ZF showed altered behavioral functions at each stage of life which emerged and attenuated as fish transitioned from each developmental stage to the next. Mitochondrial oxygen consumptions measurement results for developmentally DZN exposed ZF showed significant increases in the low and middle dose groups in organs such as the brain and testes. Overall, there is an indication that early developmental exposure to DZN had continuing adverse neurobehavioral and cellular consequences throughout their lives well into adulthood and aging periods.

有机磷 (OP) 化合物是全球使用最广泛的杀虫剂类别之一。OP 已被证明对人类健康有负面影响，特别是发育神经毒性。然而，成年后期和衰老过程中的神经毒性影响相对不具有特征。本研究检查了二嗪农 (DZN)（一种 OP），以确定除宏观尺度（整个有机体基础呼吸）和微观尺度（整个器官线粒体呼吸）的线粒体功能障碍之外的神经行为后果，使用斑马鱼 (ZF) 作为模型。一组 14 个月大的成虫 ZF 在成虫时急性暴露（0.4、1.25 和 4.0 μM）五天并作为成虫进行测试，另一组在受精后 5-120 小时（70、210、和700 nM）并在幼虫、青少年、成虫和衰老生命阶段进行测试。成人时急性接触 ZF 并没有表现出许多显着的神经行为影响或线粒体功能障碍。相反，胚胎暴露的ZF在生命的每个阶段都表现出改变的行为功能，这些改变随着鱼类从每个发育阶段过渡到下一个发育阶段而出现和减弱。发育性 DZN 暴露 ZF 的线粒体耗氧量测量结果显示，低剂量组和中剂量组的大脑和睾丸等器官的线粒体耗氧量显着增加。总体而言，有迹象表明，早期发育暴露于 DZN 会在其一生中直至成年期和衰老期持续产生不利的神经行为和细胞后果。