Objective

To assess the success rate, onset, duration and extent of motor/sensory block following neuraxial injection of two dosages of bupivacaine in bearded dragons (Pogona vitticeps).

Study design

Prospective, randomized, blinded, crossover experimental study.

Animals

A total of 10 adult bearded dragons (0.3 ± 0.1 kg).

Methods

After sedation with alfaxalone (15 mg kg^–1 subcutaneously), neuraxial injections were performed with 1 or 2 mg kg^–1 bupivacaine hydrochloride (0.5%, treatments BUP-1 and BUP-2, respectively) in a randomized treatment sequence with a 7 day washout period. If the initial bupivacaine injection was not successful within 10 minutes, a second injection was performed at the same dose. Mechanical stimulation of limbs, 25%, 50%, 75% of the trunk’s length and cloacal tone were assessed.

Results

Success rate following the first neuraxial injection was 95%, which increased to 100% after the second injection. Motor/sensory block were noted by 5 minutes after the injection of bupivacaine at either dose. BUP-2 was associated with more cranial spread. The median (range) duration of cloacal tone loss was longer following treatment BUP-2 [120 (75–225) minutes] than followed treatment BUP-1 [83 (25–135) minutes; p = 0.03]. Duration of pelvic limb motor block was comparable between both doses, lasting a median of 68 minutes in both treatments (p = 0.94). There was a transient, not clinically relevant increase from baseline in heart rate in treatment BUP-1 only. No significant difference from baseline in respiratory rate was noted in either treatment; however, two animals in treatment BUP-2 became apneic (10–20 minutes).

Conclusions and clinical relevance

Bupivacaine (1 mg kg^–1) is recommended for neuraxial anesthesia in bearded dragons. In treatment BUP-2, extensive cranial spread resulted in apnea and motor block of the thoracic limb in several animals; therefore this dose is not recommended.

中文翻译：

---

对胡须龙（Pogona vitticeps）进行布比卡因椎管内给药的评价

客观的

评估胡须龙 ( Pogona vitticeps )椎管内注射两剂布比卡因后运动/感觉阻滞的成功率、发作、持续时间和程度。

学习规划

前瞻性、随机、盲法、交叉实验研究。

动物

共有10只成年胡须龙（0.3±0.1公斤）。

方法

用阿法沙酮（15 mg kg ^–1皮下注射）镇静后，用 1 或 2 mg kg ^–1盐酸布比卡因（分别为 0.5%，治疗 BUP-1 和 BUP-2）进行椎管内注射，随机治疗顺序为 7天冲洗期。如果最初的布比卡因注射在 10 分钟内没有成功，则以相同的剂量进行第二次注射。对肢体的机械刺激、25%、50%、75%的躯干长度和泄殖腔张力进行了评估。

结果

第一次椎管内注射成功率为95%，第二次注射成功率为100%。以任一剂量注射布比卡因后 5 分钟注意到运动/感觉阻滞。BUP-2 与更多的颅内扩散有关。BUP-2 治疗后泄殖腔张力丧失的中位（范围）持续时间 [120 (75-225) 分钟] 比 BUP-1 治疗后 [83 (25-135) 分钟；p  = 0.03]。两种剂量的骨盆肢体运动阻滞持续时间相当，两种治疗的中位时间为 68 分钟（p = 0.94)。仅在治疗 BUP-1 中，心率与基线相比有短暂的、无临床相关性的增加。两种治疗均未发现呼吸频率与基线有显着差异；然而，接受 BUP-2 治疗的两只动物出现呼吸暂停（10-20 分钟）。

结论和临床相关性

布比卡因 (1 mg kg ^–1 ) 推荐用于胡须龙的椎管内麻醉。在 BUP-2 治疗中，广泛的颅骨扩散导致几只动物出现呼吸暂停和胸肢运动阻滞；因此不推荐使用此剂量。