The clinical importance of blood group (BG) antigens is related to their ability to induce immune antibodies that can cause hemolysis. Yet, ABO and D (Rh) are still considered to be the key antigens for healthy blood transfusion and secondary antigens are the next priority. Serological typing is the most widely used typing method. Rapid and accurate blood grouping plays an important role in some clinical conditions, rather than conventional techniques. Hence, developing a simple and economical model for rapid blood grouping would facilitate these tests. In recent decades, paper-based microfluidics such as μPADs has gained much interest in wide application areas such as point-of-care diagnostic. In this study, we evaluated μPADs that are performed for blood grouping and its recent progress. A comprehensive literature search was performed using databases including PUBMED, SCOPUS, Web of Science and Google Scholar. Keywords were blood grouping or typing, paper analytical device, rapid test, etc. After investigation of search results, 16 papers from 2010 to 2020 were included. Further information in detail was classified in Table 1. Generally, two principles for blood typing μPADs are introduced. The lateral chromatographic flow method and the vertical flow-through method that detects BG in a visual-based manner. To detect results with acceptable clarity many factors and challenges like paper, blood sample, buffer, Ab and RBC interaction and also μPADs stability need to be considered, which are discussed. In conclusion, the simplicity, stability, cheapness, portability and biocompatibility of μPADs for blood grouping confirming its utility and also they have the capability to robust, universal blood-grouping platform.

血型 (BG) 抗原的临床重要性与其诱导可导致溶血的免疫抗体的能力有关。然而，ABO 和 D (Rh) 仍被认为是健康输血的关键抗原，次要抗原是下一个优先事项。血清学分型是应用最广泛的分型方法。快速准确的血型分型在某些临床情况中发挥着重要作用，而不是传统技术。因此，开发一个简单且经济的快速血型模型将有助于这些测试。近几十年来，基于纸张的微流体（例如 μPAD）在医疗点诊断等广泛的应用领域引起了广泛关注。在这项研究中，我们评估了用于血型的 μPAD 及其最近的进展。使用包括 PUBMED、SCOPUS、Web of Science 和 Google Scholar 在内的数据库进行了全面的文献搜索。关键词为血型或分型、纸张分析仪、快速检测等。经检索结果调查，纳入2010-2020年的16篇论文。进一步的详细信息分类在表 1 中。通常，介绍了两种血型 μPAD 的原则。以视觉方式检测BG的横向色谱流动法和垂直流通法。为了以可接受的清晰度检测结果，需要考虑许多因素和挑战，如纸张、血样、缓冲液、Ab 和 RBC 相互作用以及 μPADs 稳定性，这些都需要加以讨论。总之，简单，稳定，便宜，