Tissues are valuable specimens in diagnostic microbiology because they are often obtained by invasive methods, and effort should thus be taken to maximize microbiological yield. The objective of this study was to evaluate the added value of using tissue pre-processing (tissue homogenizer instrument gentleMACS Dissociator) in detecting microorganisms responsible for infections. We included 104 randomly collected tissue samples, 41 (39.4 %) bones and 63 (60.6 %) soft tissues, many of those (42/104 (40.4 %)) were of periprosthetic origins. We compared the agreement between pre-processing tissues using tissue homogenizer with routine microbiology diagnostic procedure, and we calculated the performance of these methods when clinical infections were used as reference standard. There was no significant difference between the two methods (McNemar test, p = 0.3). Among the positive culture using both methods (n = 62), 61 (98.4 %) showed at least one similar microorganism. Exactly similar microorganisms were found in 42/62 (67.7 %) of the samples. From the included tissues, 55/ 104 (52.9 %) were deemed as infected. We found that the sensitivity of homogenized tissue procedure was lower (83.6 %) than when tissue was processed using tissue homogenizer (89.1 %). Sub-analysis on periprosthetic tissues and soft or bone tissues showed comparable results. The added value of GentleMACS Dissociator tissue homogenizer is limited in comparison to routine tissue processing.

中文翻译：

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使用组织匀浆器预处理组织标本：临床和微生物学评估

组织是诊断微生物学中的宝贵标本，因为它们通常是通过侵入性方法获得的，因此应努力最大限度地提高微生物产量。本研究的目的是评估使用组织预处理（组织匀浆器仪器 GentleMACS Dissociator）在检测引起感染的微生物方面的附加价值。我们纳入了 104 个随机收集的组织样本、41 个 (39.4 %) 骨骼和 63 个 (60.6 %) 软组织，其中许多 (42/104 (40.4 %)) 来自假体周围。我们比较了使用组织匀浆器预处理组织与常规微生物学诊断程序之间的一致性，并计算了当临床感染用作参考标准时这些方法的性能。两种方法之间没有显着差异（McNemar 检验，p = 0.3）。在使用两种方法的阳性培养物中 (n = 62)，61 (98.4%) 显示至少一种相似的微生物。在 42/62 (67.7%) 的样品中发现了完全相似的微生物。从所包括的组织中，55/ 104 (52.9 %) 被认为被感染。我们发现均质组织程序的灵敏度 (83.6 %) 低于使用组织均质器处理组织时 (89.1 %)。对假体周围组织和软组织或骨组织的子分析显示了可比的结果。与常规组织处理相比，GentleMACS Dissociator 组织匀浆器的附加值是有限的。9%) 被视为感染。我们发现均质组织程序的灵敏度 (83.6 %) 低于使用组织均质器处理组织时 (89.1 %)。对假体周围组织和软组织或骨组织的子分析显示了可比的结果。与常规组织处理相比，GentleMACS Dissociator 组织匀浆器的附加值是有限的。9%) 被视为感染。我们发现均质组织程序的灵敏度 (83.6 %) 低于使用组织均质器处理组织时 (89.1 %)。对假体周围组织和软组织或骨组织的子分析显示了可比的结果。与常规组织处理相比，GentleMACS Dissociator 组织匀浆器的附加值是有限的。