Wolbachia is a genus of maternally transmitted bacteria having an endosymbiotic relationship with arthropod and nematode species. These bacteria manipulate host development, sex-determination, and reproduction. In addition, they are known to potentially suppress vector-borne diseases by interfering with pathogen transmission. Although the occurrence of Wolbachia infection in insects has been known, the underlying mechanisms that mediate their interactions remain unclear. To examine the influence of Wolbachia adaptation on the host, we infected wAlbA and wAlbB strain from Aedes albopictus into the C6/36 cell line derived from Ae. albopictus. The transient Wolbachia infection was characterized by induction of cell migration without cell proliferation. The production of nitrite and reactive oxygen species (ROS) was induced by transient Wolbachia infection. Cells with transient Wolbachia infection exhibited elevated expression of Toll-like receptor 6 (TLR6) and myeloid differentiation primary response 88 (Myd88). Conversely, the expression of TLR2, TLR4, TLR7, Cactus, Ankyrin, and Argonaute2 (AGO2) was inhibited upon Wolbachia infection. These results suggest that Wolbachia has an influence on the cell migration ability as well as host innate immune response in vitro. Considering these results, transient Wolbachia strain transfer in C6/36 cells might be an important approach for studying Wolbachia-host interactions and might help gain a deeper understanding of the early adaptation of Wolbachia in the original host insect.

Wolbachia是一种母系传播的细菌，与节肢动物和线虫物种具有内共生关系。这些细菌操纵宿主发育、性别决定和繁殖。此外，众所周知，它们可以通过干扰病原体传播来潜在地抑制病媒传播疾病。尽管已知昆虫中Wolbachia感染的发生，但介导它们相互作用的潜在机制仍不清楚。为了检查沃尔巴克氏体适应对宿主的影响，我们将来自白纹伊蚊的w AlbA 和w AlbB 菌株感染到源自Ae的 C6/36 细胞系中。白线虫。短暂的Wolbachia感染的特征在于诱导细胞迁移而没有细胞增殖。亚硝酸盐和活性氧 (ROS) 的产生是由瞬时沃尔巴克氏菌感染引起的。具有瞬时沃尔巴克氏体感染的细胞表现出 Toll 样受体 6 (TLR6) 和髓样分化初级反应 88 (Myd88) 的表达升高。相反，Wolbachia感染后 TLR2、TLR4、TLR7、Cactus、Ankyrin 和 Argonaute2 (AGO2) 的表达受到抑制。这些结果表明，沃尔巴克氏体对细胞迁移能力的影响以及宿主天然免疫反应的体外。考虑到这些结果，瞬态WolbachiaC6/36 细胞中的菌株转移可能是研究Wolbachia 与宿主相互作用的重要方法，并可能有助于更深入地了解Wolbachia在原始宿主昆虫中的早期适应。