Chlorogenic acid (CGA) and its hydrolysis products, quinic and caffeic acids are considered as fine chemicals and have a high commercial value. Yerba mate stems, useless residue from yerba mate processing, contains significant amounts of CGA. The goal of this study was to determine the best conditions for maximizing the CGA aqueous extraction from residues of yerba mate processing and to fit the extraction kinetics to empirical models. The effect of single factors such as solid-liquid ratio, numbers of extraction, temperature and time were studied for two particle sizes, using one factor at a time method and Response Surface Methodology (RSM).

The highest and almost instantaneous CGA extraction (1.051 ± 0.015 g_CGA L⁻¹) was obtained using Ø particle size < 500 μm, at 65 °C, solid-liquid ratio of 1:20 and double stage extraction. For the largest particle size (2.5 × 5 mm > particle size >1 × 5 mm) the optimized conditions were 85 ± 5 °C and 25 ± 5 min, solid-liquid ratio of 1:20 and double stage extraction. For these last particles, the applied models of Pilosof et al. and Spiro and Jago showed a good agreement with the experimental and model calculated data.

In the present study, it was possible to optimize the CGA extraction conditions for two particle sizes of yerba mate stems, in order to obtain extracts with high content of CGA that can be used in the production of others fine chemicals of interest in pharmaceutical industries.

绿原酸（CGA）及其水解产物奎宁酸和咖啡酸被认为是精细化学品，具有很高的商业价值。马黛茶茎是马黛茶加工过程中的无用残留物，含有大量 CGA。本研究的目标是确定从马黛茶加工残留物中最大化 CGA 水提取的最佳条件，并使提取动力学适合经验模型。使用一次一个因子方法和响应面方法 (RSM)，研究了单一因素对两种粒径的影响，例如固液比、萃取次数、温度和时间。

使用 Ø 粒径 < 500 μm、65 °C、固液比 1:20 和双级萃取获得最高且几乎瞬时的 CGA 萃取（1.051 ± 0.015 g _CGA L ^-1）。对于最大粒径（2.5 × 5 mm > 粒径 > 1 × 5 mm），优化条件为 85 ± 5 °C 和 25 ± 5 min，固液比为 1:20，双级萃取。对于这些最后的粒子，Pilosof 等人的应用模型。Spiro 和 Jago 显示出与实验和模型计算数据的良好一致性。

在本研究中，可以优化两种粒径的马黛茶茎的 CGA 提取条件，以获得具有高 CGA 含量的提取物，可用于生产制药行业中其他感兴趣的精细化学品。