A conserved kinase domain and phosphoryl group receiver domain at the C-terminus and poly-HAMP domains at the N-terminus comprise the structural components of the group III HK which was considered as a potential antifungal target. However, the roles of individual domains in the function of group III HKs have rarely been dissected in fungi. In this study, we dissected the roles of individual domains to better understand the function of Sshk1p, a group III HK from Sclerotinia sclerotiorum. The results suggest that individual domains play different roles in the functionality of Sshk1p and are implicated in the regulation of mycelial growth, sclerotia formation, pathogenicity. And the mutants of each domain in Sshk1 showed significantly increased sensitivity to hyperosmotic stress. However, the mutants of each domain in Sshk1 showed high resistance to fludioxonil and dimethachlon which suggested that all nine domains of Sshk1p were indispensable for susceptibility to fludioxonil and dimethachlon. Moreover, deletion of each individual domain in Sshk1 cancelled intracellular glycerol accumulation and increased SsHog1p phosphorylation level triggered by NaCl and fludioxonil, suggesting that all the domains of Sshk1 were essential for Sshk1-mediated SsHog1p phosphorylation and subsequent polyol accumulation in response to fludioxonil and hyperosmotic stress.

C 端的保守激酶结构域和磷酰基受体结构域以及 N 端的聚 HAMP 结构域构成了 III HK 组的结构成分，其被认为是潜在的抗真菌靶点。然而，在真菌中很少剖析单个域在组 III HKs 的功能中的作用。在这项研究中，我们剖析了各个域的作用，以更好地了解 Sshk1p（来自核盘菌属的III 组 HK）的功能。结果表明，单个域在 Sshk1p 的功能中发挥不同的作用，并且与菌丝生长、菌核形成和致病性的调节有关。以及Sshk1中每个域的突变体对高渗应激的敏感性显着增加。然而，Sshk1中每个结构域的突变体对氟啶虫胺和敌草酮均表现出高抗性，这表明 Sshk1p 的所有 9 个结构域对于氟虫腈和敌草酮的易感性都是必不可少的。另外，在每个单独的结构域的缺失Sshk1取消细胞内甘油积累和增加了NaCl和咯菌腈触发SsHog1p磷酸化水平，这表明的所有域Sshk1分别为Sshk1介导SsHog1p磷酸化和随后的多元醇的积累必要响应于咯菌腈和高渗应激.