Because of health-promoting effects, the adaptation of skeletal muscles to exercise is considered a therapeutic strategy for metabolic complications and musculoskeletal disabilities. Myokines display many beneficial effects of different exercise modalities. Among them, irisin is known as a systemic effector that positively influences several organs. There are a few studies about the effects of irisin on skeletal muscles, and irisin prosperities need to be well-defined for being an exercise mimetic. To aim this purpose, we assessed the proteome profile of mouse skeletal muscle after eight weeks of irisin injection comparing to resistance and endurance exercise treated groups. In the current study, two-dimensional gel electrophoresis was used to evaluate the protein content of the quadriceps muscle. The results were analyzed with Image Master 2D Platinum V6 software. Differentially expressed proteins were characterized by mass spectrometry (MALDI TOF/TOF) and interpreted using protein data banks and co-expression network. Irisin increases cellular ATP content by driving its overproduction through glycolysis and oxidative phosphorylation similar to two exercise protocols and as a specific property, decreases ATP consumption through creatine kinase downregulation. It also improves the microstructural properties of quadriceps muscle by increasing fiber proteins and might induce cellular proliferation and differentiation. Network analysis of differentially expressed proteins also revealed the co-expression of Irisin precursor with structural and metabolic-related proteins. The protein alterations after irisin administration display the potential of this myokine to mimic some molecular effects of exercise, suggesting it a promising candidate to improve muscle metabolism and structure.

由于促进健康的作用，骨骼肌对运动的适应被认为是代谢并发症和肌肉骨骼残疾的治疗策略。肌动蛋白显示出不同运动方式的许多有益效果。其中，鸢尾素被认为是一种对多个器官产生积极影响的全身效应物。有一些关于鸢尾素对骨骼肌影响的研究，鸢尾素的繁荣需要明确定义为运动模拟物。为了达到这个目的，我们评估了注射鸢尾素 8 周后小鼠骨骼肌的蛋白质组谱与阻力和耐力运动治疗组相比。在目前的研究中，二维凝胶电泳用于评估股四头肌的蛋白质含量。使用 Image Master 2D Platinum V6 软件分析结果。差异表达的蛋白质通过质谱 (MALDI TOF/TOF) 进行表征，并使用蛋白质数据库和共表达网络进行解释。鸢尾素通过类似于两种运动方案的糖酵解和氧化磷酸化驱动其过度生产来增加细胞 ATP 含量，并且作为一种特定特性，通过肌酸激酶下调来减少 ATP 消耗。它还通过增加纤维蛋白来改善股四头肌的微观结构特性，并可能诱导细胞增殖和分化。差异表达蛋白的网络分析还揭示了鸢尾素前体与结构和代谢相关蛋白的共表达。