A method using UPLC-HRMS has been developed for a rapid, simultaneous qualitative and quantitative analysis of twenty-five ginsenosides. Chromatographic separation was achieved on a C₁₈ analytical column with an elution gradient comprising 0.1% aqueous formate/acetonitrile as the mobile phase. HRMS detection acquired full mass data for quantification and fullms-ddms² (i.e., data-dependent scan mode) yielded product ion spectra for identification. Furthermore, quantitative analysis of multiginsenosides by single marker (QAMS) was developed and validated using a relative correction factor. Under optimal conditions, we could simultaneously separate eight groups of isomers of the 25 ginsenosides. Good linearity was observed over the validated concentration range for each analyte (r² > 0.9924), showing excellent sensitivity (LODs, 0.003–0.349 ng/mL) and lower limit quantification (LOQs, 0.015–1.163 ng/mL). The LC-MS external standard method (ESM) and QAMS were compared and successfully applied to analyze the ginsenoside content from Panax ginseng roots. Overall, our UPLC-HRMS/QAMS approach provides high precision, stability, and reproducibility and can be used for high-throughput analysis of complex ginsenosides and quantitative analysis of multiple components and quality control of traditional Chinese medicines (TCM).

中文翻译：

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UPLC-HRMS 通过单标记物定量分析多组分同时测定 25 种人参皂苷

已开发出一种使用 UPLC-HRMS 的方法，可对 25 种人参皂苷进行快速、同步的定性和定量分析。在C ₁₈分析柱上实现色谱分离，洗脱梯度包括0.1%甲酸水溶液/乙腈作为流动相。HRMS 检测获取用于定量的全质量数据，fullms-ddms ²（即数据相关扫描模式）产生用于识别的子离子谱。此外，通过单一标记物 (QAMS) 对多人参皂苷进行定量分析，并使用相对校正因子进行了验证。在最佳条件下，我们可以同时分离出 25 种人参皂苷的 8 组异构体。在每种分析物的验证浓度范围内观察到良好的线性（r ²  > 0.9924），显示出出色的灵敏度（LOD，0.003–0.349 ng/mL）和定量下限（LOQ，0.015–1.163 ng/mL）。LC-MS 外标法 (ESM) 和 QAMS 比较并成功应用于分析人参根中的人参皂苷含量。总体而言，我们的 UPLC-HRMS/QAMS 方法具有高精度、稳定性和重现性，可用于复杂人参皂苷的高通量分析和多成分的定量分析和中药 (TCM) 的质量控制。