The purpose of this study was to investigate the treatment efficacy of recombinant Rhodococcus erythropolis (RH) expressing hydroxylamine oxidase (HAO) and ammonia monooxygenase (AMO) genes (rRH-HA) in removing nitrogen and organic matter in landfill leachate. Results demonstrated that rRH-HA efficiently expressed HAO and AMO during the treatment of landfill leachate in aerobic system. Enhanced rRH-HA production increased pH control and the activity and stability of activated sludge (AS); decreased carbon source addition and decreased total organic carbon (TOC), chemical oxygen demand (COD), organic matter and heavy metal concentration compared with AS system. In conclusion, data indicate that enhanced rRH-HA production promotes pollutants removal efficiency in the treatment of landfill leachate.

中文翻译：

---

表达HAO和AMO基因的重组红球菌促进垃圾渗滤液处理中的氮和有机物去除效率

本研究的目的是研究表达羟胺氧化酶（HAO）和氨单加氧酶（AMO）基因（rRH-HA）的重组红球菌（RH）对垃圾渗滤液中氮和有机物的去除效果。结果表明，rRH-HA在好氧系统处理垃圾渗滤液过程中有效表达HAO和AMO。增强的 rRH-HA 产生增加了 pH 控制以及活性污泥 (AS) 的活性和稳定性；与AS系统相比，碳源添加量减少，总有机碳（TOC）、化学需氧量（COD）、有机物和重金属浓度降低。总之，数据表明，提高 rRH-HA 产量可提高垃圾渗滤液处理中的污染物去除效率。