Grapevine downy mildew (DM) is a destructive oomycete disease of viticulture worldwide. MrRPV1 is a typical TIR-NBS-LRR type DM disease resistance gene cloned from the wild North American grapevine species Muscadinia rotundifolia. However, the molecular basis of resistance mediated by MrRPV1 remains poorly understood. Downy mildew-susceptible Vitis vinifera cv. Shiraz was transformed with a genomic fragment containing MrRPV1 to produce DM-resistant transgenic Shiraz lines. Comparative transcriptome analysis was used to compare the transcriptome profiles of the resistant and susceptible genotypes after DM infection. Transcriptome modulation during the response to P. viticola infection was more rapid, and more genes were induced in MrRPV1-transgenic Shiraz than in wild-type plants. In DM-infected MrRPV1-transgenic plants, activation of genes associated with Ca2+ release and ROS production was the earliest transcriptional response. Functional analysis of differentially expressed genes revealed that key genes related to multiple phytohormone signaling pathways and secondary metabolism were highly induced during infection. Coexpression network and motif enrichment analysis showed that WRKY and MYB transcription factors strongly coexpress with stilbene synthase (VvSTS) genes during defense against P. viticola in MrRPV1-transgenic plants. Taken together, these findings indicate that multiple pathways play important roles in MrRPV1-mediated resistance to downy mildew.

中文翻译：

---

转录分析揭示了抗霜霉病转基因葡萄藤的多重防御反应，该葡萄藤表达了位于 MrRUN1/MrRPV1 基因座的 TIR-NBS-LRR 基因

葡萄霜霉病 (DM) 是世界范围内葡萄栽培的破坏性卵菌病害。先生RPV1是从北美野生葡萄品种中克隆的典型TIR-NBS-LRR型DM抗病基因圆叶麝香葡萄. 然而，耐药性的分子基础由先生RPV1仍然知之甚少。霜霉病易感葡萄简历。设拉子用含有以下基因的基因组片段转化先生RPV1生产抗 DM 转基因设拉子品系。比较转录组分析用于比较 DM 感染后抗性和易感基因型的转录组谱。响应过程中的转录组调制P. viticola感染速度更快，诱导的基因更多先生RPV1-转基因设拉子比野生型植物。在 DM 感染中先生RPV1-转基因植物，激活与 Ca 相关的基因2+释放和 ROS 产生是最早的转录反应。差异表达基因的功能分析表明，与多种植物激素信号通路和次级代谢相关的关键基因在感染过程中被高度诱导。共表达网络和基序富集分析表明，WRKY 和 MYB 转录因子与二苯乙烯合酶强烈共表达。VvSTS) 防御期间的基因P. viticola在先生RPV1-转基因植物。总之，这些发现表明多种途径在MrRPV1-介导对霜霉病的抗性。