Common bean (Phaseolus vulgaris L.), one of the most consumed food legumes worldwide, is threatened by two main constraints that are found frequently together in nature, water deficit (WD) and fusarium wilt (Fop). To understand the shared and unique responses of common bean to Fop and WD, we analyzed the transcriptomic changes and phenotypic responses in two accessions, one resistant and one susceptible to both stresses, exposed to single and combined stresses. Physiological responses (photosynthetic performance and pigments quantification) and disease progression were also assessed. The combined FopWD imposition negatively affected the photosynthetic performance and increased the susceptible accession disease symptoms. The susceptible accession revealed a higher level of transcriptional changes than the resistant one, and WD single stress triggered the highest transcriptional changes. While 89 differentially expressed genes were identified exclusively in combined stresses for the susceptible accession, 35 were identified in the resistant one. These genes belong mainly to “stress”, “signaling”, “cell wall”, “hormone metabolism”, and “secondary metabolism” functional categories. Among the up-regulated genes with higher expression in the resistant accession, the cysteine-rich secretory, antigen 5 and Pr-1 (CAP) superfamily protein, a ribulose bisphosphate carboxylase family protein, and a chitinase A seem promising targets for multiple stress breeding.

中文翻译：

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共同和定制的普通豆转录组响应联合枯萎病和缺水

普通豆（菜豆L.) 是世界上消费量最大的豆类之一，受到自然界中常见的两个主要限制因素的威胁，即缺水 (WD) 和枯萎病 (福普）。了解 common bean 的共享和独特响应福普和 WD，我们分析了两种种质的转录组变化和表型反应，一种抗性和一种易受两种胁迫的影响，暴露于单一胁迫和联合胁迫。还评估了生理反应（光合性能和色素定量）和疾病进展。结合的福普WD 施加对光合性能产生负面影响并增加易感种质病症状。易感种质比抗性种质显示出更高水平的转录变化，WD 单一胁迫引发了最高的转录变化。虽然89个差异表达基因仅在敏感种质的组合胁迫中被鉴定出来，但在抗性种质中鉴定出35个。这些基因主要属于“应激”、“信号”、“细胞壁”、“激素代谢”和“二次代谢”功能类别。在抗性加入中表达较高的上调基因中，富含半胱氨酸的分泌、抗原 5 和 Pr-1 (CAP) 超家族蛋白，一种核酮糖二磷酸羧化酶家族蛋白，