Soybean varieties genetically free from Kunitz trypsin inhibitor (KTI) and lipoxygenase-2 (Lox2) are desirable to increase human consumption, as the former is an antinutritional factor that affects protein digestibility while the latter is a principal contributor to off-flavour. In the present investigation, soybean genotypes free from both these undesirable components were developed by introgression of null allele of Lox2 from NRC109 (lox2lox2) into two KTI-free soybean genotypes derived from genotypes JS97-52 and NRC7. Foreground selection of plants in F1, F2, BC1F1, BC1F2, BC2F1 and BC2F2 generations developed from two cross combinations i.e. NRC7-derived KTI-free genotype (N7KTIF)×NRC109 (parental combination 1) and JS97-52 derived KTI free genotype (JKTIF)×NRC109 (parental combination 2) was performed using null allele specific markers and tightly linked simple sequence repeat (SSR) markers for both KTI and Lox2 genes for the identification of homozygous recessive (titilox2lox2) plants. Background selection was performed using 239 and 241 polymorphic SSR markers across the genome. This resulted in the development of 9 and 13 soybean lines stacked for null alleles of both KTI and Lox2 (titilox2lox2) exhibiting recurrent parent genome content more than 97 and 96%, respectively. Days-to-flowering, days-to-maturity, 100-seed weight and yield per plant of the stacked lines developed from both the parental combinations were at par with the respective recurrent parents.

中文翻译：

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标记辅助堆积在大豆中无效的 Kunitz 胰蛋白酶抑制剂和产生异味的脂氧合酶 2

基因上不含 Kunitz 胰蛋白酶抑制剂的大豆品种（KTI) 和脂氧合酶-2 (液氧2) 是增加人类摄入量的理想选择，因为前者是影响蛋白质消化率的抗营养因子，而后者是异味的主要贡献者。在本研究中，不含这两种不良成分的大豆基因型是通过液氧2从 NRC109 (lox2lox2) 转化为来自基因型 JS97-52 和 NRC7 的两种不含 KTI 的大豆基因型。F中植物的前景选择1， F2, 公元前1F1, 公元前1F2, 公元前2F1和公元前2F2使用无效等位基因特异性标记和 JS97-52 衍生的无 KTI 基因型 (JKTIF)×NRC109（亲本组合 2），从两个交叉组合发展而来的世代，即 NRC7 衍生的无 KTI 基因型 (N7KTIF)×NRC109两者的紧密相连的简单序列重复 (SSR) 标记KTI和液氧2鉴定纯合隐性基因（titilox2lox2） 植物。使用整个基因组的 239 和 241 个多态性 SSR 标记进行背景选择。这导致开发了 9 个和 13 个大豆品系，它们的无效等位基因堆叠在一起KTI和液氧2(titilox2lox2) 分别表现出超过 97% 和 96% 的循环亲本基因组含量。由两种亲本组合开发的堆叠系的开花天数、成熟天数、100粒重和单株产量与各自的轮回亲本相当。