This study aimed to detect and identify the N-acyl-homoserine lactones molecules (AHLs) produced by different resistant Klebsiella pneumoniae isolates recovered from poultry and environmental samples using a modified validated high-performance liquid chromatography method. A total of 56 K. pneumoniae isolates were recovered, investigated for their antibiotic susceptibility, and screened for AHLs production using the Agrobacterium tumefaciens NTL4 biosensor system and a validated high-performance liquid chromatography method. The results revealed the detection of different short- and long-chain AHLs molecules among 39 K. pneumoniae isolates recovered from poultry and environmental samples. All environmental isolates produced nine peaks with retention times for C4-HSL, C6-HSL, C12-HSL, C8-HSL, C14-HSL, C8-oxo-HSL, C10-HSL, C6-oxo-HSL, and C7-HSL. The most quantifiable AHL signal molecules in poultry isolates were C4-HSL, C6-HSL, and C12-HSL. No statistical correlation between the AHL-producing ability of K. pneumoniae isolates and antibiotic resistance was reported. To the best of our knowledge, this study provides the first detailed report on the detection and identification of AHLs in K. pneumoniae isolates recovered from poultry and environmental samples. Furthermore, it provides a new insight available tool other than LC-MS/MS for detection and identification of AHL molecules.

本研究旨在使用改良的经验证的高效液相色谱方法检测和鉴定从家禽和环境样品中回收的不同耐药肺炎克雷伯菌分离株产生的N-酰基高丝氨酸内酯分子 (AHL)。总共56K 。回收肺炎分离株，研究其抗生素敏感性，并使用根癌农杆菌NTL4 生物传感器系统和经过验证的高效液相色谱方法筛选 AHL 的产生。结果显示在 39 K中检测到了不同的短链和长链 AHL 分子。从家禽和环境样本中回收的肺炎链球菌分离株。所有环境分离株均产生 9 个峰，其保留时间为 C4-HSL、C6-HSL、C12-HSL、C8-HSL、C14-HSL、C8-oxo-HSL、C10-HSL、C6-oxo-HSL 和 C7-HSL 。家禽分离物中最可量化的 AHL 信号分子是 C4-HSL、C6-HSL 和 C12-HSL。 K的 AHL 产生能力之间没有统计相关性。据报道，肺炎链球菌分离株和抗生素耐药性。据我们所知，这项研究提供了第一份关于K中 AHL 检测和鉴定的详细报告。从家禽和环境样本中回收的肺炎链球菌分离株。此外，它还为 AHL 分子的检测和鉴定提供了除 LC-MS/MS 之外的新的可用工具。