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Osteoblastic response to biomaterials surfaces: Extracellular matrix proteomic analysis
Journal of Biomedical Materials Research Part B: Applied Biomaterials ( IF 3.2 ) Pub Date : 2021-07-01 , DOI: 10.1002/jbm.b.34900
Marcia Sirlene Zardin Graeff 1 , Cintia Kazuko Tokuhara 2 , Mariana Liessa Rovis Sanches 2 , Marília Afonso Rabelo Buzalaf 2 , Luis Augusto Rocha 3, 4 , Rodrigo Cardoso de Oliveira 2, 4
Affiliation  

The cellular response to surfaces is mediated, among other factors, by the extracellular matrix (ECM). However, little is known about the ECM proteome during mineralization. Our objective was to compare the ECM composition formed by osteoblast on different materials surfaces with proteomic analysis. Three types of biomaterial surfaces (pure titanium, anodized titanium, and zirconia) were used. Osteoblasts (MC3T3 linage) cells were cultivated on the biomaterials for 7, 14, and 21 days with the osteogenic medium. For the proteomic analysis, the specimens were washed, decellularized, and the ECM was collected. The majority of the typical ECM proteins, out of a total of 24 proteins identified, was expressed and regulated equally on the three biomaterials tested. Alpha-1,4 glucan phosphorylase was found to be down-regulated on zirconia on the seventh day, while at the same time, glycogen phosphorylase brain form was up-regulated on anodized titanium, both when compared with pure titanium (ratio: 1.06 and 0.97, respectively). And after 14 days of culture, glycogen phosphorylase brain form was downregulated on zirconia when compared with pure titanium (ratio: 0.90), suggesting the influence of material surface roughness and chemical composition on energy metabolism. Proteins related to bone development like Transforming growth factor beta-3 and Fibroblast growth factor 8 were found exclusively on pure titanium on the 21st day. Altogether, our results show a possible influence of material surfaces on the composition of ECM.

中文翻译:

成骨细胞对生物材料表面的反应:细胞外基质蛋白质组学分析

除其他因素外,细胞对表面的反应由细胞外基质 (ECM) 介导。然而,关于矿化过程中的 ECM 蛋白质组知之甚少。我们的目标是通过蛋白质组学分析比较成骨细胞在不同材料表面形成的 ECM 成分。使用了三种类型的生物材料表面(纯钛、阳极氧化钛和氧化锆)。用成骨培养基在生物材料上培养成骨细胞(MC3T3 系)细胞 7、14 和 21 天。对于蛋白质组学分析,清洗样本、去细胞化并收集 ECM。在鉴定的总共 24 种蛋白质中,大多数典型的 ECM 蛋白质在所测试的三种生物材料上均等地表达和调节。阿尔法-1,与纯钛相比,氧化锆上的 4 葡聚糖磷酸化酶在第 7 天下调,而同时,糖原磷酸化酶脑型在阳极氧化钛上上调(比率分别为 1.06 和 0.97) . 培养14天后,与纯钛相比,氧化锆上的糖原磷酸化酶脑型下调(比率:0.90),表明材料表面粗糙度和化学成分对能量代谢的影响。第 21 天,仅在纯钛上发现了与骨骼发育相关的蛋白质,如转化生长因子 β-3 和成纤维细胞生长因子 8。总之,我们的结果显示了材料表面对 ECM 组成的可能影响。与纯钛相比,阳极氧化钛上的糖原磷酸化酶脑型上调(比率分别为 1.06 和 0.97)。培养14天后,与纯钛相比,氧化锆上的糖原磷酸化酶脑型下调(比率:0.90),表明材料表面粗糙度和化学成分对能量代谢的影响。第 21 天,仅在纯钛上发现了与骨骼发育相关的蛋白质,如转化生长因子 β-3 和成纤维细胞生长因子 8。总之,我们的结果显示了材料表面对 ECM 组成的可能影响。与纯钛相比,阳极氧化钛上的糖原磷酸化酶脑型上调(比率分别为 1.06 和 0.97)。培养14天后,与纯钛相比,氧化锆上的糖原磷酸化酶脑型下调(比率:0.90),表明材料表面粗糙度和化学成分对能量代谢的影响。第 21 天,仅在纯钛上发现了与骨骼发育相关的蛋白质,如转化生长因子 β-3 和成纤维细胞生长因子 8。总之,我们的结果显示了材料表面对 ECM 组成的可能影响。与纯钛相比,氧化锆上的糖原磷酸化酶脑型下调(比率:0.90),表明材料表面粗糙度和化学成分对能量代谢的影响。第 21 天,仅在纯钛上发现了与骨骼发育相关的蛋白质,如转化生长因子 β-3 和成纤维细胞生长因子 8。总之,我们的结果显示了材料表面对 ECM 组成的可能影响。与纯钛相比,氧化锆上的糖原磷酸化酶脑型下调(比率:0.90),表明材料表面粗糙度和化学成分对能量代谢的影响。第 21 天,仅在纯钛上发现了与骨骼发育相关的蛋白质,如转化生长因子 β-3 和成纤维细胞生长因子 8。总之,我们的结果显示了材料表面对 ECM 组成的可能影响。
更新日期:2021-07-01
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