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Isolation of Plant Growth Promoting Rhizobacteria from Spartina densiflora and Sarcocornia perennis in San Antonio polluted salt marsh, Patagonian Argentina
Estuarine, Coastal and Shelf Science ( IF 2.6 ) Pub Date : 2021-07-01 , DOI: 10.1016/j.ecss.2021.107488
Yanina L. Idaszkin , Rosana Polifroni , Jennifer Mesa-Marín

With the purpose of Plant Growth Promoting Rhizobacteria (PGPR) isolation, several authors have sampled different wetlands in the northern half of Argentina. However, to date, we do not know the existence of microbiological studies conducted in the Patagonian salt marshes, and concretely, concerning isolation of PGPR. The present work was conducted in the heavy metal polluted San Antonio salt marsh, in two areas with different grade of pollution. In those areas, we isolated cultivable bacteria from the rhizosphere of the halophytes Spartina densiflora and Sarcocornia perennis, and look for several plant growth promoting (PGP) properties among them. In total, 60 different cultivable bacteria were isolated. 50% of the rhizobacterial strains demonstrated at least one of the PGP properties assayed, 25% of them produced siderophores, 16% were able to solubilize phosphate, 11% were able to produce auxins and 7% chitinase. We could observe that PGP properties were more abundant among bacteria growing in polluted soils. Also, bacteria inhabiting S. densiflora rhizosphere showed more PGP properties related to heavy metal phytostabilization mechanisms, in line with the phytoremediation strategy of this halophyte. Overall, these findings support the idea that coastal hazardous scenarios may be a good opportunity to seek for PGPR. Indeed, some of the strains isolated in this work presented more than one PGP property, so they may be selected for further formulation of inoculants for different applications. For further research, it would be interesting to analyse other PGP properties in these strains, as well as to isolate rhizobacteria from other halophytes in diverse Patagonian salt marshes.



中文翻译:

从阿根廷巴塔哥尼亚圣安东尼奥污染盐沼中的密花马齿苋和多年生肉豆蔻中分离促进植物生长的根际细菌

为了分离促进植物生长的根际细菌 (PGPR),几位作者对阿根廷北半部的不同湿地进行了采样。然而,迄今为止,我们不知道是否存在在巴塔哥尼亚盐沼中进行的微生物研究,具体而言,关于 PGPR 的分离。目前的工作是在重金属污染的圣安东尼奥盐沼中进行的,在两个污染等级不同的地区进行。在这些地区,我们从盐生植物Spartina densifloraSarcocornia perennis的根际分离出可培养的细菌,并在其中寻找几种植物生长促进 (PGP) 特性。总共分离出 60 种不同的可培养细菌。50% 的根际细菌菌株表现出至少一种 PGP 特性,其中 25% 产生铁载体,16% 能够溶解磷酸盐,11% 能够产生生长素和 7% 几丁质酶。我们可以观察到,在污染土壤中生长的细菌中,PGP 特性更为丰富。此外,居住在S. densiflora 的细菌根际表现出更多与重金属植物稳定机制相关的 PGP 特性,符合该盐生植物的植物修复策略。总体而言,这些发现支持这样一种观点,即沿海危险情景可能是寻求 PGPR 的好机会。事实上,在这项工作中分离的一些菌株表现出不止一种 PGP 特性,因此可以选择它们来进一步配制用于不同应用的接种剂。对于进一步的研究,分析这些菌株的其他 PGP 特性,以及从不同巴塔哥尼亚盐沼中的其他盐生植物中分离根际细菌会很有趣。

更新日期:2021-07-07
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