Abstract

Due to urbanization, automation and mechanization of labor, as well as difficult epidemiological situation and self-isolation, the level of daily physical activity in a modern man decreases. The aim of the study was to study the effect of restricted activity on protein synthesis in postural and locomotor muscles. An experiment with activity restricted for 21 days was carried out on Wistar rats. The intensity of protein synthesis and anabolic signaling pathways was studied in the soleus muscle (m. soleus) mainly consisting of slow fibers, and the extensor digitorum longus muscle (m. EDL), mainly consisting of fast fibers. The mass of m. soleus and m. EDL was reduced, while a significant decrease in protein synthesis was observed only in m. EDL. There was also a decrease in the phosphorylation of S6 ribosomal protein only in the fast muscle. At the same time, in contrast to m. EDL, а GSK3β phosphorylation was decreased in m. soleus. Additionally, the markers of proteolysis were studied. In the experiment, a decrease in the expression of MuRF-1 in m. soleus and Atrogin-1/MAFbx in m. EDL was observed, as well as an increase in the expression of calpains in m. soleus. Thus, atrophic processes caused by restricted activity in fast and slow muscles are determined by different signaling mechanisms.

中文翻译：

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姿势和运动肌肉中的受限活动和蛋白质合成

摘要

由于城市化、劳动力的自动化和机械化，以及困难的流行病和自我隔离，现代人的日常体育活动水平下降。该研究的目的是研究限制活动对姿势肌肉和运动肌肉中蛋白质合成的影响。对 Wistar 大鼠进行了一项活动受限 21 天的实验。在主要由慢纤维组成的比目鱼肌（比目鱼肌）和主要由快纤维组成的趾长伸肌（m. EDL）中研究了蛋白质合成和合成代谢信号通路的强度。m的质量。比目鱼和米。EDL减少，而仅在m中观察到蛋白质合成显着减少。EDL。仅在快速肌肉中，S6 核糖体蛋白的磷酸化也有所降低。同时，与m相反。EDL , а GSK3β 磷酸化在m中降低。比目鱼。此外，研究了蛋白水解的标志物。在实验中，MuRF-1在m中的表达降低。比目鱼和 Atrogin-1/MAFbx 以米为单位。观察到EDL，以及m中钙蛋白酶的表达增加。比目鱼。因此，由快速和慢速肌肉的活动受限引起的萎缩过程是由不同的信号机制决定的。