Abstract

In this study, the composition of the essential oil of tea tree (Melaleuca alternifolia) leaves and the antifungal effects of the essential oil on food-borne molds isolated from meat products were investigated. The α-pinene (21.64 %), γ-terpinene (21.09 %), terpinen-4-ol (17.31 %), limonene (9.37 %), and o-cymene (6.54 %) were found to be the major components of M. alternifolia essential oil. The essential oil showed the highest antifungal activity on Aspergillus niger (25.82 mm zone diameter), followed by Aspergillus fumigatus (22.27 mm), Aspergillus flavus (21.092 mm), and Penicillium notatum (20.30 mm) (p<0.05), respectively. The minimum inhibitory concentration (MIC) values of M. alternifolia essential oil on the mold species used in the study varied between 0.032 and 0.376 mg/L, and the value of minimum fungicidal concentration (MFC) values ranged from 0.021 to 0.341 mg/L (p<0.05). The MIC/MFC ratio of M. alternifolia essential oil on 10 different mold species varies between 1.05 and 1.68 mg/L. According to the MIC/MFC ratio, the M. alternifolia essential oil showed a lethal effect on all of the mold species studied in this research.

摘要

在这项研究中，研究了茶树（互叶白千层）叶精油的成分以及精油对从肉制品中分离出的食源性霉菌的抗真菌作用。发现 α-蒎烯 (21.64%)、γ-萜品烯 (21.09%)、萜品烯-4-醇 (17.31%)、苎烯 (9.37%) 和邻散花烃 (6.54%) 是M的主要成分. 互叶花精油。精油对黑曲霉（区域直径为 25.82 毫米）的抗真菌活性最高，其次是烟曲霉（22.27 毫米）、黄曲霉（21.092 毫米）和青霉（20.30 毫米）（p < 0.05）。最小抑菌浓度 (MIC) 值M. alternifolia精油对研究中使用的霉菌种类在 0.032 至 0.376 mg/L 之间变化，最低杀菌浓度 (MFC) 值的范围为 0.021 至 0.341 mg/L (p<0.05)。M. alternifolia精油对 10 种不同霉菌的 MIC/MFC 比率在 1.05 和 1.68 mg/L 之间变化。根据 MIC/MFC 比率，互叶梅精油对本研究中研究的所有霉菌物种均显示出致死作用。