Abstract

Hyaluronic acid-based nanofiber scaffolds imitating the natural extracellular matrix, which is promising for use in tissue engineering, have been prepared by electrospinning. Hyaluronic acid is part of many organs and tissues; it is a biologically active component capable of being involved in cell proliferation and migration. The high viscosity of solutions of the macromolecular biopolymer significantly complicates the preparation of nanofiber scaffolds based on it. Rheological studies have made it possible to determine the solvent composition that contributes to a decrease in the viscosity of the spinning solution. The highest Newtonian viscosity of a 1% hyaluronic acid solution in water is 25 Pa s; in the case of using a mixture of ammonia hydrate (10%) and dimethylformamide at a ratio of 2 : 1, this parameter decreases to 1.147 Pa s. The use of a special spinning cell with a small-diameter nozzle and a high pressure (up to 10 atm) has provided the formation of hyaluronic acid-based nanofibers with a diameter of 100–300 nm.

中文翻译：

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静电纺丝法制备高分子透明质酸纳米纤维材料

摘要

已经通过静电纺丝制备了基于透明质酸的纳米纤维支架，模仿天然细胞外基质，有望用于组织工程。透明质酸是许多器官和组织的一部分；它是一种能够参与细胞增殖和迁移的生物活性成分。高分子生物聚合物溶液的高粘度使基于它的纳米纤维支架的制备显着复杂化。流变学研究使得确定有助于降低纺丝溶液粘度的溶剂组成成为可能。1%透明质酸水溶液的最高牛顿粘度为25Pa·s；在使用比例为 2:1 的氨水 (10%) 和二甲基甲酰胺的混合物的情况下，该参数降低到 1.147 Pa s。