Tomato brown rugose fruit virus (ToBRFV) causes severe fruit loss in tomato (Solanum lycopersicum) and pepper (Capsicum annuum) plants. It is an emerging Tobamovirus that is spreading globally. The major challenge is to develop a reliable method for the detection of the virus, and to better characterize the symptoms it causes. The aims of this study, therefore, were to characterize the symptom development on tomato and pepper plants, and to establish a reliable detection method for the virus. Following infection of the tomato and pepper plants with ToBRFV, the leaves turned chlorotic, mosaic or mottled, while the fruit became rugose, necrotic and marbled, and showed discoloration with yellow or brown spots. Transmission electron microscopy (TEM) revealed single rod-like virus particles characteristic of the Tobamoviruses. Classical reverse transcription PCR (RT-PCR) and quantitative PCR (qPCR) with specific primers and probes confirmed that the virus is ToBRFV. We found that the resistance genes from tomato, Tm-2², and pepper, L1, L2, L3 and L4, did not confer resistance to ToBRFV. Here, we present a PCR-based method as a diagnostic test for detecting ToBRFV in infected seeds. This method will help to prevent further spread of the virus in commercial seeds.

番茄褐色皱纹果实病毒 (ToBRFV) 导致番茄 ( Solanum lycopersicum ) 和辣椒 ( Capsicum annuum ) 植物的严重落果。它是一种新兴的烟草病毒这正在全球蔓延。主要的挑战是开发一种可靠的方法来检测病毒，并更好地表征病毒引起的症状。因此，本研究的目的是表征番茄和辣椒植物的症状发展，并建立一种可靠的病毒检测方法。ToBRFV感染番茄和辣椒植株后，叶片褪绿、花叶或斑驳，而果实则有皱纹、坏死和大理石纹，并出现黄色或褐色斑点。透射电子显微镜 (TEM) 揭示了托巴莫病毒特有的单个杆状病毒颗粒。使用特定引物和探针的经典逆转录 PCR (RT-PCR) 和定量 PCR (qPCR) 证实该病毒为 ToBRFV。我们发现，来自番茄的抗性基因，TM-2 2 ，和胡椒，L1，L2，L3和L4，没有赋予对ToBRFV。在这里，我们提出了一种基于 PCR 的方法作为检测受感染种子中 ToBRFV 的诊断测试。这种方法将有助于防止病毒在商业种子中进一步传播。