This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P < 0.01). HE staining showed HDPCs grew in multilayer within the scaffolds. CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs. CSF scaffolds were promising candidates in dentine-pulp complex regeneration.

中文翻译：

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不同孔径I型胶原/丝素蛋白支架对人牙髓细胞增殖分化的影响

本研究旨在评估不同孔径大小的 I 型胶原蛋白/丝素蛋白 (CSF) 支架对人牙髓细胞 (HDPCs) 增殖和分化的影响。CSF 支架是使用 3D 绘图软件 Solidworks 设计的。通过低温沉积 3D 打印技术制备了三种不同孔径尺寸的支架 (CSF1-CSF3)。通过扫描电子显微镜（SEM）和光学相干断层扫描观察形态。分别检测支架的孔隙率、亲水性和机械能力。将 HDPC（第三代，1×105 细胞）接种到每个支架中，并通过 SEM、CCK-8、碱性磷酸酶（ALP）活性和 HE 染色进行研究。CSF支架具有具有大孔和微孔的多孔结构。CSF1 至 CSF3 的大孔径为 421 ± 27 μm，分别为 579 ± 36 μm 和 707 ± 43 μm。孔隙率分别为 69.8 ± 2.2%、80.1 ± 2.8% 和 86.5 ± 3.3%。所有这些支架都增强了 HDPC 的粘附和增殖。CSF1组的ALP活性高于CSF3组（P＜0.01）。HE染色显示HDPCs在支架内多层生长。CSF支架显着提高了HDPCs的粘附和ALP活性。CSF 支架是牙本质-牙髓复合物再生的有希望的候选者。CSF支架显着提高了HDPCs的粘附和ALP活性。CSF 支架是牙本质-牙髓复合物再生的有希望的候选者。CSF支架显着提高了HDPCs的粘附和ALP活性。CSF 支架是牙本质-牙髓复合物再生的有希望的候选者。