An efficient and rapid liquid chromatographic method was developed for the separation of carotenoids and their geometrical isomers in tomato products using a core C30 column of 2.6 μm particles with gradient elution of tert-butyl-methyl-ether in 2% water in methanol. Excellent separation of the major carotenoids such as lycopene, β-carotene and lutein as well as their geometrical isomers and oxygen-containing derivatives with resolution factors ranging between 0.78 and 4.0 and selectivity of 1.01–1.63 was achieved. Validation of the developed method met the acceptance criteria concerning linearity, recovery, precision and limit of detection and quantification. Calibrations were linear with correlation coefficient (R2) values between 0.9966 and 0.9999. The limit of detection and quantification values were found to be 0.008 and 0.017 and 0.029 and 0.056 μg/mL, respectively. Recovery of 94.3–99.9%, intraday precision of 1.81–4.45% and interday precision of 3.13–6.86% were obtained. The hyphenation of liquid chromatography with diode-array and mass spectrometry was helpful in the identification of the separated carotenoids particularly the unusual di-hydroxy cyclolycopene adduct and di-methoxy lycopene determined for the first time in tomato products. Commercially available kinds of tomato juice and ketchup were evaluated based on their carotenoid content.

中文翻译：

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在 Core-C30 色谱柱上进行高效 HPLC 分离，对番茄产品中的异构体、衍生物和异常类胡萝卜素进行 MS2 表征

开发了一种高效、快速的液相色谱方法，用于分离番茄制品中的类胡萝卜素及其几何异构体，该方法使用 2.6 μm 颗粒的 C30 芯柱，在甲醇中的 2% 水的叔丁基甲基醚梯度洗脱。实现了对主要类胡萝卜素（如番茄红素、β-胡萝卜素和叶黄素）及其几何异构体和含氧衍生物的出色分离，分离因子在 0.78 和 4.0 之间，选择性在 1.01-1.63 之间。所开发方法的验证符合有关线性、回收率、精密度以及检测限和定量限的验收标准。校准是线性的，相关系数 (R2) 值介于 0.9966 和 0.9999 之间。检测限和定量值分别为 0.008 和 0.017 以及 0.029 和 0。分别为 056 μg/mL。回收率为 94.3-99.9%，日内精度为 1.81-4.45%，日间精度为 3.13-6.86%。液相色谱与二极管阵列和质谱联用有助于鉴定分离的类胡萝卜素，特别是在番茄制品中首次确定的不寻常的二羟基环番茄红素加合物和二甲氧基番茄红素。根据其类胡萝卜素含量评估市售种类的番茄汁和番茄酱。液相色谱与二极管阵列和质谱联用有助于鉴定分离的类胡萝卜素，特别是在番茄制品中首次确定的不寻常的二羟基环番茄红素加合物和二甲氧基番茄红素。根据其类胡萝卜素含量评估市售种类的番茄汁和番茄酱。液相色谱与二极管阵列和质谱联用有助于鉴定分离的类胡萝卜素，特别是在番茄制品中首次确定的不寻常的二羟基环番茄红素加合物和二甲氧基番茄红素。根据其类胡萝卜素含量评估市售种类的番茄汁和番茄酱。