This work reports the enantioresolution of (RS)-acebutolol (ACB) by a thin-layer chromatographic method involving colistin sulfate (CS) as chiral selector. For this purpose, glass plates of dimension (12 cm × 4 cm × 0.4 cm) were prepared and used in four different approaches. The chiral selector was used in four different approaches before or during the chromatographic processes. Out of several mobile phase systems tried, the mobile phase system MeCN–MeOH−H₂O (5.5:2.5:1.0, V/V) was found to be successful for better resolution (R_S = 2.2). To confirm the elution order, the chromatograms of (RS)-ACB were developed along with the pure enantiomer of ACB, i.e., (R)-ACB, and it was concluded that (R)-ACB eluted before (S)-enantiomer. The detection limit for each enantiomer of ACB was found in the range 12−15 μg spot⁻¹. The developed method can be applied for the enantioresolution of ACB and its analogs in dosage formulations.

这项工作报告了使用硫酸粘菌素 (CS) 作为手性选择剂的薄层色谱法对 ( RS )-醋丁洛尔 (ACB) 进行对映拆分。为此，制备了尺寸（12 cm × 4 cm × 0.4 cm）的玻璃板，并以四种不同的方法使用。在色谱过程之前或期间，手性选择器用于四种不同的方法。在尝试的几种流动相系统中，发现流动相系统 MeCN–MeOH-H ₂ O (5.5:2.5:1.0, V/V ) 成功获得更好的分辨率 ( R _S = 2.2)。为了确认洗脱顺序，( RS )-ACB的色谱图与 ACB 的纯对映异构体一起开发，即(R )-ACB，并得出结论，( R )-ACB 在 ( S )-对映异构体之前洗脱。ACB 的每个对映异构体的检测限在 12-15 μg 点^-1的范围内。所开发的方法可用于制剂中 ACB 及其类似物的对映拆分。