The nuclear factor Y (NF-Y) transcription factor (TF) family consists of three subfamilies NF-YA, NF-YB and NF-YC. Many studies have proven that NF-Y complex plays multiple essential roles in stress response in Arabidopsis and other plant species. However, little attention has been given to these genes in peanut. In this study, thirty-three AhNF-Y genes were identified in cultivated peanut and they were distributed on 16 chromosomes. A phylogenetic analysis of the NF-Y amino acid sequences indicated that the peanut NF-Y proteins were clustered in pairs at the end of the branches and showed high conservation with previous reported plant NF-Ys. Evolutionary history analysis showed that only segmental duplication contributed to expansion of this gene family. Analysis of the 1500-bp regulatory regions upstream the start codon showed that, except for AhNF-YB6, peanut NF-Ys contained at least one abiotic stress response element in their regulatory region. Expression patterns of peanut NF-Ys in 22 tissues and developmental stages were analyzed. A few NF-Ys showed universal expression patterns, while most NF-Ys showed specific expression patterns. Through RNA-seq and qRT-PCR analyses, expression of six AhNF-Y genes was induced under salt stress in leaves or roots. In addition, AhNF-YA4/8/11, NF-YB4 and NF-YC2/8 also responded to osmotic stress, ABA (abscisic acid) and salicylic acid (SA) treatment.

核因子 Y (NF-Y) 转录因子 (TF) 家族由三个亚家族 NF-YA、NF-YB 和 NF-YC 组成。许多研究已经证明 NF-Y 复合物在拟南芥和其他植物物种的胁迫响应中起着多种重要作用。然而，很少有人关注花生中的这些基因。在这项研究中，三十三个AhNF-Y在栽培花生中鉴定出基因，它们分布在16条染色体上。NF-Y 氨基酸序列的系统发育分析表明，花生 NF-Y 蛋白在分枝末端成对聚集，与先前报道的植物 NF-Y 具有高度保守性。进化史分析表明，只有节段重复有助于该基因家族的扩展。对起始密码子上游 1500 bp 调控区的分析表明，除AhNF-YB6 外，花生NF-Y s 在其调控区中至少含有一种非生物胁迫响应元件。分析了花生NF-Ys在 22 个组织和发育阶段中的表达模式。几个NF-Ys 显示出普遍的表达模式，而大多数NF-Y s 显示出特定的表达模式。通过 RNA-seq 和 qRT-PCR 分析，在盐胁迫下叶或根中诱导了 6 个 AhNF-Y 基因的表达。此外，AhNF-YA4 / 8 / 11、NF-YB4和NF-YC2 / 8也响应渗透胁迫、ABA（脱落酸）和水杨酸（SA）处理。